Obesity and malnutrition similarly alter the renin–angiotensin system and inflammation in mice and human adipose
| Autor: | Mariane Bertagnolli, Alfredo Maurício Batista de Paula, Deborah de Farias Lelis, André Luiz Sena Guimarães, Sérgio Henrique Sousa Santos, Thales de Almeida Pinheiro, Gislaine Candida Batista-Jorge, João Marcus Oliveira Andrade, Emillio Cesar Neves Ferreira, Carlos Juliano Brant Albuquerque, Alanna Fernandes Paraíso, Antônio Prates Caldeira, Thaísa Soares Crespo, Thaisa de Almeida Pinheiro, Ugo Borges Pinheiro, Antônio Sérgio Barcala-Jorge, Cássio André Vieira |
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| Rok vydání: | 2017 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine Panniculitis Tecido adiposo Endocrinology Diabetes and Metabolism Clinical Biochemistry Adipose tissue Sistema renina-angiotensina Biochemistry Renin-Angiotensin System Mice chemistry.chemical_compound Adipocyte Aged 80 and over Nutrition and Dietetics Middle Aged Cholesterol Obesidade Distúrbios alimentares Female Tumor necrosis factor alpha medicine.symptom Adult medicine.medical_specialty Intra-Abdominal Fat Inflammation Desnutrição Biology Proinflammatory cytokine Dieta de baixa caloria 03 medical and health sciences Insulin resistance Internal medicine medicine Animals Humans Obesity Molecular Biology Aged Malnutrition medicine.disease 030104 developmental biology Endocrinology chemistry Case-Control Studies Insulin Resistance Biomarkers |
| Zdroj: | Repositório Institucional da UFMG Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/j.jnutbio.2017.06.008 |
| Popis: | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Outra Agência The main goal of the present study was to evaluate the metabolic profile, inflammatory markers and the gene expression of the renin–angiotensin system (RAS) components in the visceral adipose tissue of eutrophic, obese and malnourished individuals and mice models of obesity and food restriction. Male Swiss mice were divided into eight groups and fed different levels of food restriction (20%, 40%, or 60%) using standard or high-fat diet. Metabolic profile and adipose tissues were assessed. The expression of AGT (Angiotensinogen), ACE (Angiotensin-converting enzyme), ACE2 (Angiotensin-converting enzyme 2), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in the mice epididymal adipose tissue and the human visceral adipose tissue was assessed. The main findings showed reduced body weight, improved metabolism, decreased adipose tissues weight and reduced adipocyte area in mice submitted to food restriction. Diminished expression of IL-6, TNF-α, AGT, AT1 and ACE was detected in the 20% and 40% food restriction animal groups, although they were increased in the 60% malnourished group. Increased expression of IL-6, TNF-α, AGT and ACE in obese and malnourished individuals was observed. Adipocytes size was increased in obese individuals and reduced in malnutrition. In conclusion, we found that food restriction of 20% and 40% improved the metabolic profile, ameliorated the inflammatory status and down-regulated the RAS in mice. Severe 60% food restriction (malnutrition), however, stimulated a proinflammatory state and increased AGT and ACE expression in the adipose tissue of mice. A similar profile was observed in the adipose tissue of obese and malnourished humans, supporting the critical role of inflammation and RAS as mediators of metabolic disorders. |
| Databáze: | OpenAIRE |
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