Effects of dietary restriction on induction of unscheduled DNA synthesis (UDS) and replicative DNA synthesis (RDS) in rat liver
| Autor: | Chie Furihata, Hirohiko Daimon, Kazuaki Ogura, Kiyomi Yamatsu, Taneo Fukuda, Shigeki Sawada, Shoji Asakura |
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| Rok vydání: | 1994 |
| Předmět: |
DNA Replication
Male medicine.medical_specialty DNA Repair CCL4 Biology Toxicology Dimethylnitrosamine S Phase chemistry.chemical_compound In vivo Oral administration Internal medicine Demethylase activity Genetics medicine Animals Carbon Tetrachloride Cells Cultured DNA synthesis Organ Size Molecular biology Rats Inbred F344 Rats Endocrinology medicine.anatomical_structure Liver chemistry Nitrosamine Hepatocyte Microsomes Liver Energy Intake Thymidine |
| Zdroj: | Mutation Research/Genetic Toxicology. 322:257-264 |
| ISSN: | 0165-1218 |
| DOI: | 10.1016/0165-1218(94)90101-5 |
| Popis: | The effects of dietary restriction on the induction of unscheduled DNA synthesis (UDS) and replicative DNA synthesis (RDS) were studied in the hepatocytes of F344 rats exposed in vivo to dimethylnitrosamine (DMN) or CCl4. The animals were given food ad libitum, a restricted amount of food (4 g/rat/overnight) or no food. Hepatocytes were isolated 2 h after oral administration of DMN at a dose of 5 mg/kg body weight and 48 h after oral administration of CCl4 at a dose of 400 mg/kg body weight, and incubated for 4 h in Williams' medium E supplemented with either [3H]thymidine for UDS or 5-bromodeoxyuridine for RDS. UDS was determined by autoradiography and RDS was determined by the immunoenzymatic staining method. The background levels of UDS (net grains/nucleus) and RDS (cells in S phase) in control were −12.4 and 0.64% for ad libitum feeding, −6.8 and 0.04% for restricted feeding, and −8.1 and 0% for fasting. UDS induced by DMN and RDS induced by CCl4 were 19.4 and 3.3% for ad libitum feeding, 34.5 and 10.4% for restricted feeding, and 47.8 and 15.1% for fasting. DMN demethylase activity in rat liver was also found to increase with dietary restriction. These results indicate that dietary restriction modulates the responses of UDS and RDS in the liver of rats. |
| Databáze: | OpenAIRE |
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