Automated rare single cell picking with the ALS cellcelector™
| Autor: | Constantin Nelep, Jens Eberhardt |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Histology Computer science automated image analysis Single tumor Cell Cell Culture Techniques Cell Count Cell Separation Computational biology single cell picking circulating tumor cells imaging cytometry Pathology and Forensic Medicine Brain cancer 03 medical and health sciences Circulating tumor cell Cell Line Tumor Neoplasms medicine Humans Liquid biopsy liquid biopsy Amyotrophic Lateral Sclerosis Reproducibility of Results Cell Biology Neoplastic Cells Circulating purification of CTCs Molecular analysis 030104 developmental biology medicine.anatomical_structure Cell culture CTC isolation CTC Technologies and Tools Single-Cell Analysis rare single cells automated cell micromanipulation Cytometry rare cell isolation |
| Zdroj: | Cytometry |
| ISSN: | 1552-4930 1552-4922 |
| DOI: | 10.1002/cyto.a.23568 |
| Popis: | Molecular analysis of rare single cells like circulating tumor cells (CTCs) from whole blood patient samples bears multiple challenges. One of those challenges is the efficient and ideally loss‐free isolation of CTCs over contaminating white and red blood cells. While there is a multitude of commercial and non‐commercial systems available for the enrichment of CTCs their cell output does not deliver the purity most molecular analysis methods require. Here we describe the ALS CellCelector™ which can solve this challenge allowing the retrieval of 100% pure single CTCs from blood processed by different upstream enrichment techniques. It is a multifunctional, extremely flexible system for automated screening of cell culture plates, Petri dishes, and microscope slides. Fixed or live single cells or multicellular clusters detected during screening can be picked out of those plates automatically. The complete scan and picking process is fully documented hence allowing highest standardization and reproducibility of all processes. Use of CellCelector allowed the isolation of pure single tumor cells or clusters from liquid biopsies of breast, prostate, ovarian, colorectal, lung, and brain cancers for their subsequent molecular analysis. © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. |
| Databáze: | OpenAIRE |
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