Construction of a male sterility system for hybrid rice breeding and seed production using a nuclear male sterility gene
| Autor: | Zhenyi Chang, Tang Xiaoyan, Xing Wang Deng, Zhufeng Chen, Junli Zhou, Na Wang, Gang Xie, Wei Yan, Jiawei Lu |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Tapetum Multidisciplinary Oryza sativa Sterility Software maintainer food and beverages Genetically modified crops Biological Sciences Biology medicine.disease_cause Corrections 01 natural sciences Hybrid seed Pollen exine formation 03 medical and health sciences 030104 developmental biology Pollen Botany medicine 010606 plant biology & botany |
| Zdroj: | Proceedings of the National Academy of Sciences. 113:14145-14150 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The breeding and large-scale adoption of hybrid seeds is an important achievement in agriculture. Rice hybrid seed production uses cytoplasmic male sterile lines or photoperiod/thermo-sensitive genic male sterile lines (PTGMS) as female parent. Cytoplasmic male sterile lines are propagated via cross-pollination by corresponding maintainer lines, whereas PTGMS lines are propagated via self-pollination under environmental conditions restoring male fertility. Despite huge successes, both systems have their intrinsic drawbacks. Here, we constructed a rice male sterility system using a nuclear gene named Oryza sativa No Pollen 1 (OsNP1). OsNP1 encodes a putative glucose-methanol-choline oxidoreductase regulating tapetum degeneration and pollen exine formation; it is specifically expressed in the tapetum and miscrospores. The osnp1 mutant plant displays normal vegetative growth but complete male sterility insensitive to environmental conditions. OsNP1 was coupled with an α-amylase gene to devitalize transgenic pollen and the red fluorescence protein (DsRed) gene to mark transgenic seed and transformed into the osnp1 mutant. Self-pollination of the transgenic plant carrying a single hemizygous transgene produced nontransgenic male sterile and transgenic fertile seeds in 1:1 ratio that can be sorted out based on the red fluorescence coded by DsRed Cross-pollination of the fertile transgenic plants to the nontransgenic male sterile plants propagated the male sterile seeds of high purity. The male sterile line was crossed with ∼1,200 individual rice germplasms available. Approximately 85% of the F1s outperformed their parents in per plant yield, and 10% out-yielded the best local cultivars, indicating that the technology is promising in hybrid rice breeding and production. |
| Databáze: | OpenAIRE |
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