Measurement of lung fluid volumes and albumin exclusion in sheep
| Autor: | Robert J. Roselli, R. E. Parker, Thomas R. Harris, N. A. Pou, J.A. Clanton |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
Physiology Hematocrit Iodine Radioisotopes Albumins Physiology (medical) Technetium-99 medicine Animals Lung volumes Respiratory system Lung Distribution Volume Sheep Isotopes of chromium Chromatography medicine.diagnostic_test business.industry Chemistry Lethal dose Albumin Technetium Pentetic Acid Female Lymph Extracellular Space Nuclear medicine business |
| Zdroj: | Journal of Applied Physiology. 67:1323-1330 |
| ISSN: | 1522-1601 8750-7587 |
| Popis: | A radioactive tracer technique was used to determine interstitial diethylenetriaminepentaacetic acid (DTPA) and albumin distribution volume in sheep lungs. {sup 125}I- and/or {sup 131}I-labeled albumin were injected intravenously and allowed to equilibrate for 24 h. {sup 99m}Tc-labeled DTPA and {sup 51}Cr-labeled erythrocytes were injected and allowed to equilibrate (2 h and 15 min, respectively) before a lethal dose of thiamylal sodium. Two biopsies (1-3 g) were taken from each lung and the remaining tissue was homogenized for wet-to-dry lung weight and volume calculations. Estimates of distribution volumes from whole lung homogenized samples were statistically smaller than biopsy samples for extravascular water, interstitial {sup 99m}Tc-DTPA, and interstitial albumin. The mean fraction of the interstitium (Fe), which excludes albumin, was 0.68 +/- 0.04 for whole lung samples compared with 0.62 +/- 0.03 for biopsy samples. Hematocrit may explain the consistent difference. To make the Fe for biopsy samples match that for homogenized samples, a mean hematocrit, which was 82% of large vessel hematocrit, was required. Excluded volume fraction for exogenous sheep albumin was compared with that of exogenous human albumin in two sheep, and no difference was found at 24 h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|