Microbial biodiversity of the liquid fraction of rumen content from lactating cows
| Autor: | Chiara Manfrin, Alberto Pallavicini, Bruno Stefanon, Misa Sandri |
|---|---|
| Přispěvatelé: | Sandri, M, Manfrin, Chiara, Pallavicini, Alberto, Stefanon, B. |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Next-Generation Sequencing
Gram-Positive Endospore-Forming Rods 16S Rumen Firmicutes Volatile rumen bacteria community Saccharomyces cerevisiae Biology SF1-1100 RNA Ribosomal 16S Animals Lactation dairy cows Microbiome Food science 16S rRNA Animal Husbandry Biodiversity Cattle Dairying Diet Fatty Acids Volatile Female Milk Animal Science and Zoology Medicine (all) Ribosomal dairy cow Fatty Acids food and beverages Ribosomal RNA 16S ribosomal RNA biology.organism_classification Yeast Animal culture Metagenomics RNA Composition (visual arts) |
| Zdroj: | Animal, Vol 8, Iss 4, Pp 572-579 (2014) |
| Popis: | Host and dietary interactions with the rumen microbiome can affect the efficacy of supplements, and their effect on the composition of the bacterial population is still unknown. A 16S rRNA metagenomic approach and Next-Generation Sequencing (NGS) technology were used to investigate the bacterial microbiome composition in the liquid fraction of the rumen content collected via stomach tubing. To investigate biodiversity, samples were taken from three groups of four lactating dairy cows given a supplement of either 50 g of potato protein (Ctrl group), or 50 g of lyophilized Saccharomyces cerevisiae (LY group) or 50 g of dried S. cerevisiae (DY group) in a potato protein support. Rumen samples were collected after 15 days of dietary treatments and milk production was similar between the three groups. Taxonomic distribution analysis revealed a prevalence of the Firmicutes phylum in all cows (79.76%) and a significantly (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|