High-Density Single Antibody Electrochemical Nanoarrays
| Autor: | Khalil Chennit, Yannick Coffinier, Shuo Li, Nicolas Clément, Agnès Anne, Arnaud Chovin, Christophe Demaille |
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| Přispěvatelé: | Laboratoire d'Electrochimie Moléculaire (LEM (UMR_7591)), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), This work has benefited from the facilities and expertise of the Mass Spectrometry platform of ICSN, Institut de Chimie des Substances Naturelle (Vincent Guérineau, CNRS, Gif-sur-Yvette, France, http://icsn.cnrs.fr). |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry
[CHIM.ANAL]Chemical Sciences/Analytical chemistry [CHIM]Chemical Sciences General Materials Science [CHIM.CATA]Chemical Sciences/Catalysis Electrical and Electronic Engineering Condensed Matter Physics Atomic and Molecular Physics and Optics |
| Zdroj: | Nanoresearch Nanoresearch, In press, ⟨10.1007/s12274-022-5137-1⟩ |
| Popis: | International audience; The fabrication and electrochemical interrogation of very high density single-antibody nanoarrays is reported. Gold nanodots, 15 nm in diameter, arranged in large (cm2) square arrays with a pitch of 200 nm, are used as carriers for primary antibodies (Immunoglobulin G, IgGs), further recognized by secondary redox-labeled detection antibodies. Ensemble scale interrogation of the antibody array by cyclic voltammetry, and nanoscale interrogation of individual nanodots by mediator tethered atomic-force electrochemical microscopy (Mt/AFM-SECM), enable the occupancy of nanodots by single antibody molecules to be demonstrated. Experiments involving the competitive adsorption of antibodies of different species onto the nanodots evidence the possibility of using single-antibody nanoarrays for digital electrochemical immunoassays. |
| Databáze: | OpenAIRE |
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