Supplemental Figures 1 - 9, Table 1, Methods from CCL5-Mediated Th2 Immune Polarization Promotes Metastasis in Luminal Breast Cancer

Autor: Wei-Qiang Gao, Yan Zhang, Bing Zhang, Lei Gong, Lin Zhong, Jilong Qin, Qianfei Zhang
Rok vydání: 2023
Popis: Figure S1. Analysis of IL4 expresion of CD4+ T cells and the number of CTC at different time points in MMTV-PyMT mice. Figure S2. Analysis of tumor-associated myeloid cells in MMTV-PyMT mice. Figure S3. Efficient in vivo depletion of CD4+ T cells following i. v. injection of anti-CD4 antibody (GK1.5) and analysis of tumor-associated myeloid cells in CCL5-/- PyMT mice after depletion of CD4+ T cells. Figure S4. Confirmation of the epithelial origin of MECs collected from the 3D-organoid co-culture. Figure S5. The expression of EMT-related genes in caner cells from CCL5+/+ PyMT and CCL5-/- PyMT mice. Figure S6. Protein level of CCL5 in different cell types of MMTV-PyMT mice. Figure S7. Quantification of Western Blotting data of Fig 5B and 5C. Figure S8. Efficient infection of lentivirus encoding Gfi1. Figure S9. Expression pattern of CCL5 receptors in CCL5+/+ PyMT mice. Figure S10. CCL5 and IL4 expression are not associated with the subtype of human luminal breast cancer. Percentage of IL4+CD4+ T cells in PBMC of luminal breast cancer patients. Table S1. Antibodies used in the paper.
Databáze: OpenAIRE