Novel quercetin encapsulated chitosan functionalized copper oxide nanoparticles as anti-breast cancer agent via regulating p53 in rat model
| Autor: | Nemany A.N. Hanafy, Naglaa M. Sherif, Nahla S. Hassan, Awny M. Elsayed, Fayez Althobaiti, Heba A. Sahyon |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Programmed cell death
Cell Survival 9 10-Dimethyl-1 2-benzanthracene Drug Compounding Metal Nanoparticles Caspase 3 Antineoplastic Agents Breast Neoplasms 02 engineering and technology Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology In vivo Proliferating Cell Nuclear Antigen medicine Animals Humans Molecular Biology 030304 developmental biology Cell Proliferation 0303 health sciences Chitosan biology Cytochrome c Cell Cycle Cancer General Medicine Hep G2 Cells 021001 nanoscience & nanotechnology medicine.disease Xenograft Model Antitumor Assays In vitro Rats Gene Expression Regulation Neoplastic chemistry Apoptosis Cancer research biology.protein MCF-7 Cells Female Quercetin Caco-2 Cells Tumor Suppressor Protein p53 0210 nano-technology Copper |
| Zdroj: | International journal of biological macromolecules. 185 |
| ISSN: | 1879-0003 |
| Popis: | This study was designed to present a new quercetin encapsulated chitosan functionalized copper oxide nanoparticle (CuO-ChNPs-Q) and assessed its anti-breast cancer activity both in vitro and in vivo. The CuO-ChNPs-Q may act as anti-proliferating agent against DMBA-induced mammary carcinoma in female rats. The CuONPs was functionalized with chitosan then quercetin was conjugated with them producing CuO-ChNPs-Q, then characterized. The in vitro anti-proliferating activity of the CuO-ChNPs-Q was evaluated against three human cell line. Then, the anti-breast cancer effect of the CuO-ChNPs-Q was assessed against DMBA-induction compared to both CuONPs and Q in female rat model. The in vitro results proved the potent anticancer activity of the CuO-ChNPs-Q compared to CuONPs and quercetin. The in vivo data showed significant reduction in breast tumors of DMBA-induced rats treated with CuO-ChNPs-Q compared to CuONPs and Q. The CuO-ChNPs-Q treatment had induced apoptosis via increased p53 gene, arrested the cell-cycle, and increased both cytochrome c and caspase-3 levels leading to mammary carcinoma cell death. Also, the CuO-ChNPs-Q treatment had suppressed the PCNA gene which decreased the proliferation of the mammary carcinoma cells. In conclusion, the CuO-ChNPs-Q might be a promising chemotherapeutic agent for treatment of breast cancer with a minimal toxicity on vital organs. |
| Databáze: | OpenAIRE |
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