Expression analysis and prognostic significance of human kallikrein 11 in prostate cancer
Autor: | Panagiota Stavropoulou, Mario Plebani, Andreas Scorilas, Alkiviades K. Gregorakis |
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Rok vydání: | 2005 |
Předmět: |
Male
Gene isoform PCA3 Clinical Biochemistry Biology Biochemistry Prostate cancer Prostate medicine Humans Aged Neoplasm Staging Gene Expression Profiling Biochemistry (medical) Prostatic Neoplasms KLK5 Cancer General Medicine Kallikrein Middle Aged Prostate-Specific Antigen Prognosis medicine.disease Molecular biology Gene Expression Regulation Neoplastic Prostate-specific antigen medicine.anatomical_structure Cancer research Kallikreins |
Zdroj: | Clinica Chimica Acta. 357:190-195 |
ISSN: | 0009-8981 |
Popis: | Background Kallikrein 11 ( KLK11 ) is a newly discovered human kallikrein gene that is mainly expressed in the central nervous system and endocrine tissues. KLK11 has two alternative splicing isoforms, known as the brain type and prostate type. Many members of the human kallikrein gene family are differentially expressed in cancer and a few have potential as diagnostic/prognostic markers. Methods In the present study, the expression of prostate type variant of KLK11 gene was analyzed by RT-PCR in 66 prostate cancer tissues. Tumors were pulverized, total RNA was extracted, and cDNA was prepared by reverse transcription. KLK11 was amplified by PCR using gene specific primers and its identity was verified by sequencing. Prostate tissues were then classified as KLK5 positive or negative based on eithidium bromide staining in agarose gels and image analysis. Results KLK11 was found to be highly expressed in 43/66 (65%) of prostate cancer samples. We found a significant negative relationship between KLK11 expression and Gleason score ( p = 0.004) and disease stage ( p = 0.038). Serum total PSA concentration was found to be lower in patients with overexpression of KLK11 ( p = 0.044). Conclusions We conclude that down-regulation of the KLK11 gene in advanced and more aggressive tumors may open the possibility of being used as a future biological marker distinguishing the tumor aggressiveness as well as a useful prognostic biomarker for prostate cancer. |
Databáze: | OpenAIRE |
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