Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages
| Autor: | Aude A. Sauvage, Benoît Bihin, Karim Zouaoui Boudjeltia, Valérie Pireaux, Alexandre Rousseau, Pierre Van Antwerpen, Martine Raes, Martine Van Steenbrugge |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antioxidant Article Subject medicine.medical_treatment Immunology Chimie analytique Macrophage polarization Stimulation Sciences biomédicales en général Marker gene 03 medical and health sciences medicine lcsh:Pathology ARG1 Chimie pharmaceutique Gene biology Cell Biology Phenotype Cell biology 030104 developmental biology Myeloperoxidase biology.protein lipids (amino acids peptides and proteins) Sciences pharmaceutiques Research Article lcsh:RB1-214 |
| Zdroj: | Mediators of inflammation, 2016 Mediators of Inflammation, Vol 2016 (2016) Mediators of Inflammation |
| Popis: | Macrophages and oxidized LDLs play a key role in atherogenesis but their heterogeneity has been neglected up to now. Macrophages are prone to polarization and subsets of polarized macrophages have been described in atheromas. LDLs can be oxidized not only chemically by copper (Ox-LDLs) but also enzymatically by myeloperoxidase (MpOx-LDLs) resulting in oxidized LDLs poor in lipid peroxides. The effects of physiologically relevant myeloperoxidase-oxidized LDLs on macrophage polarization or on polarized macrophages remain largely unknown. In this study, the effects of LDLs on macrophage polarization were investigated by monitoring the expression of M1 and M2 genes following stimulation with native LDLs, Ox-LDLs, or MpOx-LDLs in RAW 264.7 cells. Except for MRC1, which is induced only by Ox-LDLs, MpOx-LDLs induced an overexpression of most of the selected marker genes at the mRNA level. MpOx-LDLs also modulate marker gene expression in polarized macrophages favoring notably anti-inflammatory Arg1 expression in M2 cells and also in the other phenotypes. Noteworthy, MpOx-LDLs were the most efficient to accumulate lipids intracellularly in (un)polarized macrophages whatever the phenotype. These data were largely confirmed in murine bone marrow-derived macrophages. Our data suggest that MpOx-LDLs were the most efficient to accumulate within cells and to enhance an anti-inflammatory and antioxidant phenotype in M2 cells and also in the other macrophage phenotypes. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|