Enzymatic Hydrolysis of Tetriso as a Model for the Detoxication of the Neurotoxic Agent VX
| Autor: | J. E. Walker, F. C. G. Hoskin |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Chromatography
Hydrolases Organothiophosphorus Compounds Cell analysis Biology Models Biological Sperm Detoxication chemistry.chemical_compound Tissue culture Organophosphorus Compounds Human fertilization chemistry Pseudomonas Enzymatic hydrolysis biology.animal Inactivation Metabolic embryonic structures Cholinesterase Inhibitors General Agricultural and Biological Sciences Hydrogen peroxide Sea urchin |
| Zdroj: | The Biological Bulletin. 187:249-250 |
| ISSN: | 1939-8697 0006-3185 |
| DOI: | 10.1086/bblv187n2p249 |
| Popis: | Figure 1. Effects of N-monomethyl-L-arginine on hydrogen peroxide,formation during the fertilization of sea urchin eggs. Eggs, immobilized on poly-L-lysine coated tissue culture plates, were incubatedfor 30 min with DCFH-DA (5 @I), incubated with sperm, and, after 10 min. analyzed with a Meridian ACAS anchored cell analysis system. Representative images are presented. Panel A, untreated,fertilized eggs; panel B, eggs treated with 1 mA4 L-arginine; panel C, eggs treated with I mA4 NMMA; panel D, eggs treated with I mM NMMA in the presence of 1 mM L-arginine. The color bar represents increasing fluorescence on a fourdecade log scale. Each panel shows a representative,fieldfrom 12 different scans |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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