Human AML cells in NOD/SCID mice: engraftment potential and gene expression
| Autor: | Rui Zheng, Baldwin Br, Norbert Claude Gorin, Gerard Hoehn, Curt I. Civin, Douglas R. Smith, Matthew T Malehorn, Donald Small, Steven D. Gore, Meltzer Ps, Rachata Lumkul |
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| Rok vydání: | 2002 |
| Předmět: |
Receptors
CXCR4 Cancer Research DNA Complementary Microarray Ratón Transplantation Heterologous Mice SCID Nod Biology Flow cytometry Mice Antigens CD Bone Marrow Mice Inbred NOD Proto-Oncogene Proteins hemic and lymphatic diseases Tumor Cells Cultured medicine Animals Humans Oligonucleotide Array Sequence Analysis medicine.diagnostic_test Serial Transplantation Gene Expression Profiling Graft Survival Receptor Protein-Tyrosine Kinases Myeloid leukemia Hematology medicine.disease Transplantation Disease Models Animal Leukemia fms-Like Tyrosine Kinase 3 Oncology Leukemia Myeloid Acute Disease Immunology Cell Division Neoplasm Transplantation |
| Zdroj: | Leukemia. 16:1818-1826 |
| ISSN: | 1476-5551 0887-6924 |
| DOI: | 10.1038/sj.leu.2402632 |
| Popis: | Most cases of human acute myeloid leukemia (AML) engraft in irradiated non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice. Intravenous transfer of as few as 10(5) human AML cells resulted in engraftment. Cases with poor prognosis clinical features, including FLT3 mutations, tended to engraft efficiently. Nevertheless, AML cells obtained from patients at relapse did not engraft more efficiently than cells obtained from the same patients at initial diagnosis. One passage of human AML cells in NOD/SCID mice did not appear to select for increased virulence, as measured by serial transplantation efficiency. Finally, cDNA microarray analyses indicated that approximately 95% of genes were expressed at similar levels in human AML cells immunopurified after growth in mice, as compared to cells assessed directly from patients. Thus, the growth of human AML cells in NOD/SCID mice could yield large numbers of human AML cells for direct experimental use and could also function as a renewable, potentially unlimited source of leukemia cells, via serial transplantation. |
| Databáze: | OpenAIRE |
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