Genome-Wide Association Study Using Extreme Truncate Selection Identifies Novel Genes Affecting Bone Mineral Density and Fracture Risk
| Autor: | Kari Stefansson, Rajesh V. Thakker, Mark A. Kotowicz, J. B. Richards, Richard L. Prince, John D. Wark, K. Pryce, Cyrus Cooper, Johanna Hadler, S.C. Nguyen, Karol Estrada, K. Addison, Elaine M. Dennison, Margaret J. Henry, John P. Kemp, David M. Reid, Julie A. Pasco, Christopher T. Esapa, David M. Evans, Eugene V. McCloskey, Graeme Jones, P. N. Sambrook, Matthew A. Brown, Albert Hofman, Tuan V. Nguyen, Unnur Styrkarsdottir, Patrick Danoy, Linda A. Bradbury, Joanna Makovey, Gudmar Thorleifsson, R. Tichawangana, Claus-Christian Glüer, Dieter Felsenberg, Tim D. Spector, Fernando Rivadeneira, André G. Uitterlinden, Emma L. Duncan, Paul Leo, John A. Eisman, R. Eastell, Steve D.M. Brown, C. Roux, Roger D. Cox, G.C. Nicholson, Ian R. Reid, C. Cremin |
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| Přispěvatelé: | Internal Medicine, Epidemiology, Pediatric Surgery, Family Medicine, RS: CAPHRI School for Public Health and Primary Care |
| Rok vydání: | 2011 |
| Předmět: |
Male
Cancer Research Bone density Osteoporosis Genome-wide association study QH426-470 Diabetes and Endocrinology/Bone and Mineral Metabolism Bioinformatics Cohort Studies Fractures Bone Mice 0302 clinical medicine Bone Density Chromosomes Human Osteoporosis Postmenopausal Genetics and Genomics/Genetics of Disease Genetics (clinical) Aged 80 and over Genetics 0303 health sciences education.field_of_study Rheumatology/Bone and Mineral Metabolism Middle Aged 3. Good health Models Animal N-Acetylgalactosaminyltransferases Female Proteoglycans Research Article musculoskeletal diseases Genotype Population 030209 endocrinology & metabolism Single-nucleotide polymorphism Biology Quantitative trait locus Polymorphism Single Nucleotide SOXC Transcription Factors 03 medical and health sciences Chloride Channels medicine Animals Humans Integrin-Binding Sialoprotein education Molecular Biology Ecology Evolution Behavior and Systematics Aged 030304 developmental biology Genetic association Bone fracture medicine.disease Disease Models Animal Latent TGF-beta Binding Proteins Case-Control Studies Mutation Thrombospondins Receptors Transforming Growth Factor beta Developmental Biology Genome-Wide Association Study |
| Zdroj: | PLoS Genetics (print), 7(4). Public Library of Science PLoS Genetics, Vol 7, Iss 4, p e1001372 (2011) PLoS Genetics; Vol 7 PLoS Genetics Plos Genetics, 7(4):e1001372. Public Library of Science |
| ISSN: | 1553-7404 1553-7390 |
| DOI: | 10.1371/journal.pgen.1001372 |
| Popis: | Osteoporotic fracture is a major cause of morbidity and mortality worldwide. Low bone mineral density (BMD) is a major predisposing factor to fracture and is known to be highly heritable. Site-, gender-, and age-specific genetic effects on BMD are thought to be significant, but have largely not been considered in the design of genome-wide association studies (GWAS) of BMD to date. We report here a GWAS using a novel study design focusing on women of a specific age (postmenopausal women, age 55–85 years), with either extreme high or low hip BMD (age- and gender-adjusted BMD z-scores of +1.5 to +4.0, n = 1055, or −4.0 to −1.5, n = 900), with replication in cohorts of women drawn from the general population (n = 20,898). The study replicates 21 of 26 known BMD–associated genes. Additionally, we report suggestive association of a further six new genetic associations in or around the genes CLCN7, GALNT3, IBSP, LTBP3, RSPO3, and SOX4, with replication in two independent datasets. A novel mouse model with a loss-of-function mutation in GALNT3 is also reported, which has high bone mass, supporting the involvement of this gene in BMD determination. In addition to identifying further genes associated with BMD, this study confirms the efficiency of extreme-truncate selection designs for quantitative trait association studies. Author Summary Osteoporotic fracture is a major cause of early mortality and morbidity in the community. To identify genes associated with osteoporosis, we have performed a genome-wide association study. In order to improve study power and to address the demographic group of highest risk from osteoporotic fracture, we have used a unique study design, studying 1,955 postmenopausal women with either extreme high or low hip bone mineral density. We then confirmed our findings in 20,898 women from the general population. Our study replicated 21 of 26 known osteoporosis genes, and it identified a further six novel loci (in or nearby CLCN7, GALNT3, IBSP, LTBP3, RSPO3, and SOX4). For one of these loci, GALTN3, we demonstrate in a mouse model that a loss-of-function genetic mutation in GALNT3 causes high bone mass. These findings report novel mechanisms by which osteoporosis can arise, and they significantly add to our understanding of the aetiology of the disease. |
| Databáze: | OpenAIRE |
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