Posttranscriptional gene silencing in nuclei
| Autor: | Toni Voelker, Sergey I. Ivashuta, Alexa Vitins, Craig S. Pikaard, Nicholas L. Wagner, Andrew Mroczka, Olga Pontes, Paul Hoffer |
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| Rok vydání: | 2010 |
| Předmět: |
Fatty Acid Desaturases
Ribonuclease III RNA-induced transcriptional silencing RNA-induced silencing complex Immunoblotting Trans-acting siRNA Arabidopsis Biology RNA interference Gene expression RNA Precursors Gene silencing RNA Small Interfering Cell Nucleus Multidisciplinary Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction fungi Biological Sciences RNA-Dependent RNA Polymerase Molecular biology Introns RNA silencing Microscopy Fluorescence RNA Interference Soybeans Small nuclear RNA |
| Zdroj: | Proceedings of the National Academy of Sciences. 108:409-414 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1009805108 |
| Popis: | In plants, small interfering RNAs (siRNAs) with sequence homology to transcribed regions of genes can guide the sequence-specific degradation of corresponding mRNAs, leading to posttranscriptional gene silencing (PTGS). The current consensus is that siRNA-mediated PTGS occurs primarily in the cytoplasm where target mRNAs are localized and translated into proteins. However, expression of an inverted-repeat double-stranded RNA corresponding to the soybean FAD2-1A desaturase intron is sufficient to silence FAD2-1 , implicating nuclear precursor mRNA (pre-mRNA) rather than cytosolic mRNA as the target of PTGS. Silencing FAD2-1 using intronic or 3′-UTR sequences does not affect transcription rates of the target genes but results in the strong reduction of target transcript levels in the nucleus. Moreover, siRNAs corresponding to pre-mRNA–specific sequences accumulate in the nucleus. In Arabidopsis, we find that two enzymes involved in PTGS, Dicer-like 4 and RNA-dependent RNA polymerase 6, are localized in the nucleus. Collectively, these results demonstrate that siRNA-directed RNA degradation can take place in the nucleus, suggesting the need for a more complex view of the subcellular compartmentation of PTGS in plants. |
| Databáze: | OpenAIRE |
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