Identification and functional analysis of NLP-encoding genes from the postharvest pathogen penicillium expansum
| Autor: | Samir Droby, Oleg Feygenberg, Michael Wisniewski, Radi Aly, Ana-Rosa Ballester, Jing Ma, Ginat Raphael, Luis González-Candelas, John L. Norelli, Elena Levin |
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| Přispěvatelé: | Israel Science Foundation, National Natural Science Foundation of China |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Microbiology (medical) Virulence Nicotiana benthamiana apple Blue mold computer.software_genre 01 natural sciences Microbiology Article 03 medical and health sciences Virology Nep1 pathogenicity Pathogenicity blue mold Gene Pathogen lcsh:QH301-705.5 030304 developmental biology 0303 health sciences biology business.industry Effector fungi Apple food and beverages biology.organism_classification Effectors virulence lcsh:Biology (General) Postharvest Artificial intelligence Penicillium expansum business computer Natural language processing 010606 plant biology & botany effectors |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Microorganisms, Vol 7, Iss 6, p 175 (2019) Microorganisms Volume 7 Issue 6 |
| Popis: | © The Author(s). Penicillium expansum is a major postharvest pathogen that infects different fruits, mainly through injuries inflicted during harvest or subsequent handling after harvest. Several effectors were suggested to mediate pathogenicity of P. expansum in fruit tissue. Among these effectors Nep1-like proteins (NLPs), produced by various microorganisms with different lifestyles, are known for their ability to induce necrosis in dicot plants and were shown to be involved in virulence of several plant-related pathogens. This study was aimed at the identification and functional characterization of two NLP genes found in the genome of P. expansum. The genes were designated Penlp1 and Penlp2 and were found to code type1 and type3 NLP respectively. Necrosis-inducing activity of the two proteins was demonstrated by transient expression in Nicotiana benthamiana leaves. While Penlp1 expression was induced during apple infection and in liquid culture, the highest level of Penlp2 expression was found in ungerminated spores. Deletion of Penlp1, but not Penlp2, resulted in reduced virulence on apples manifested by reduced rate of lesion development (disease severity). This research was funded by Israel Science Foundation (ISF) and National Natural Science Foundation of China (NSFC), 1936/14, awarded to Samir Droby. |
| Databáze: | OpenAIRE |
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