Downregulation of the E2 Subunit of 2-Oxoglutarate Dehydrogenase Modulates Plant Growth by Impacting Carbon-Nitrogen Metabolism in Arabidopsis thaliana
| Autor: | Adriano Nunes-Nesi, Alisdair R. Fernie, Jonas Rafael Vargas, Jorge Luis Pérez-Díaz, Jorge A Condori-Apfata, Willian Batista-Silva, David B. Medeiros, Wagner L. Araújo, Luiz Mário Lopes Valente |
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| Rok vydání: | 2020 |
| Předmět: |
Physiology
Nitrogen Protein subunit Mutant Arabidopsis Down-Regulation Dehydrogenase Germination Primary metabolism Plant Science AcademicSubjects/SCI01180 Gene Expression Regulation Plant Regular Paper Arabidopsis thaliana Ketoglutarate Dehydrogenase Complex Photosynthesis TCA cycle Phylogeny chemistry.chemical_classification biology AcademicSubjects/SCI01210 Respiration food and beverages 2-OGDH Cell Biology General Medicine Metabolism biology.organism_classification Carbon Amino acid Citric acid cycle Protein Subunits Biochemistry chemistry Seedlings Seeds |
| Zdroj: | Plant and Cell Physiology |
| ISSN: | 1471-9053 |
| Popis: | In Arabidopsis thaliana, two genes encode the E2 subunit of the 2-oxoglutarate dehydrogenase (2-OGDH), a multimeric complex composed of three subunits. To functionally characterize the isoforms of E2 subunit, we isolated Arabidopsis mutant lines for each gene encoding the E2 subunit and performed a detailed molecular and physiological characterization of the plants under controlled growth conditions. The functional lack of expression of E2 subunit isoforms of 2-OGDH increased plant growth, reduced dark respiration and altered carbohydrate metabolism without changes in the photosynthetic rate. Interestingly, plants from e2-ogdh lines also exhibited reduced seed weight without alterations in total seed number. We additionally observed that downregulation of 2-OGDH activity led to minor changes in the levels of tricarboxylic acid cycle intermediates without clear correlation with the reduced expression of specific E2-OGDH isoforms. Furthermore, the e2-ogdh mutant lines exhibited a reduction by up to 25% in the leaf total amino acids without consistent changes in the amino acid profile. Taken together, our results indicate that the two isoforms of E2 subunit play a similar role in carbon–nitrogen metabolism, in plant growth and in seed weight. |
| Databáze: | OpenAIRE |
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