Sarcomere Stiffness during Stretching and Shortening of Rigor Skeletal Myofibrils
| Autor: | Alf Månsson, Dilson E. Rassier, Malin Persson, Nabil Shalabi, Srikar Vengallatore |
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| Rok vydání: | 2017 |
| Předmět: |
Sarcomeres
0301 basic medicine Materials science 030102 biochemistry & molecular biology Atomic force microscopy Biophysics Stiffness Skeletal muscle Microscopy Atomic Force Sarcomere Viscoelasticity Biomechanical Phenomena Cytoskeletal Proteins 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure medicine Animals Female Molecular Machines Motors and Nanoscale Biophysics Rabbits Composite material medicine.symptom Myofibril Mechanical Phenomena |
| Zdroj: | Biophysical Journal |
| ISSN: | 0006-3495 |
| Popis: | In this study, we measured the stiffness of skeletal muscle myofibrils in rigor. Using a custom-built atomic force microscope, myofibrils were first placed in a rigor state then stretched and shortened at different displacements (0.1–0.3 μm per sarcomere) and nominal speeds (0.4 and 0.8 μm/s). During stretching, the myofibril stiffness was independent of both displacement and speed (average of 987 nN/μm). During shortening, the myofibril stiffness was independent of displacement, but dependent on speed (1234 nN/μm at 0.4 μm/s; 1106 nN/μm at 0.8 μm/s). Furthermore, the myofibril stiffness during shortening was greater than that during stretching and the difference depended on speed (31% at 0.4 μm/s; 8% at 0.8 μm/s). The results suggest that the myofibrils exhibit nonlinear viscoelastic properties that may be derived from myofibril filaments, similar to what has been observed in muscle fibers. |
| Databáze: | OpenAIRE |
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