SPTBN1 suppresses the progression of epithelial ovarian cancer via SOCS3-mediated blockade of the JAK/STAT3 signaling pathway
Autor: | Mo Chen, Xiuling Zhi, Huijie Wu, Jiajia Li, Xuhui Dong, Liangqing Yao, Yuan Lei, Jia Zeng, Shuyi Chen |
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Rok vydání: | 2020 |
Předmět: |
STAT3 Transcription Factor
epithelial ovarian cancer Aging Epithelial-Mesenchymal Transition endocrine system diseases Mice Nude Apoptosis Vimentin Stat3 Signaling Pathway Mice Downregulation and upregulation Cell Line Tumor JAK/STAT pathway Animals Humans SOCS3 Cell Proliferation Ovarian Neoplasms biology Chemistry Mesenchymal stem cell EMT Spectrin JAK-STAT signaling pathway Cell Biology Janus Kinase 2 Xenograft Model Antitumor Assays female genital diseases and pregnancy complications Gene Expression Regulation Neoplastic Suppressor of Cytokine Signaling 3 Protein Cell culture Disease Progression Cancer research biology.protein Immunohistochemistry Female Signal Transduction Research Paper SPTBN1 |
Zdroj: | Aging (Albany NY) |
ISSN: | 1945-4589 |
Popis: | SPTBN1 plays an anticancer role in many kinds of tumors and participates in the chemotherapeutic resistance of epithelial ovarian cancer (EOC). Here, we reported that lower SPTBN1 expression was significantly related to advanced EOC stage and shorter progression-free survival. SPTBN1 expression was also higher in less invasive EOC cell lines. Moreover, SPTBN1 decreased the migration ability of the EOC cells A2780 and HO8910 and inhibited the growth of EOC cells in vitro and tumor xenografts in vivo. SPTBN1 suppression increased the epithelial mesenchymal transformation marker Vimentin while decreasing E-cadherin expression. By analyzing TCGA data and immunohistochemistry staining of tumor tissue, we found that SPTBN1 and SOCS3 were positively coexpressed in EOC patients. SOCS3 overexpression or JAK2 inhibition decreased the proliferation and migration of EOC cells as well as the expression of p-JAK2, p-STAT3 and Vimentin, which were enhanced by the downregulation of SPTBN1, while E-cadherin expression was also reversed. It was also verified in mouse embryonic fibroblasts (MEFs) that loss of SPTBN1 activated the JAK/STAT3 signaling pathway with suppression of SOCS3. Our results suggest that SPTBN1 suppresses the progression of epithelial ovarian cancer via SOCS3-mediated blockade of the JAK/STAT3 signaling pathway. |
Databáze: | OpenAIRE |
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