Adhesive Properties and Hydrolytic Enzymes of Oral Candida albicans Strains
Autor: | Rafael Sentandreu, Lucas del Castillo, Hajer Hentati, Eulogio Valentín, Emira Noumi, Kacem Mahdouani, Mejdi Snoussi, Amina Bakhrouf |
---|---|
Rok vydání: | 2009 |
Předmět: |
Adult
Veterinary (miscellaneous) medicine.medical_treatment Virulence Applied Microbiology and Biotechnology Microbiology Fungal Proteins Candida albicans Cell Adhesion medicine Humans Stomatitis Aged chemistry.chemical_classification Mouth Protease biology Biofilm Middle Aged biology.organism_classification medicine.disease Stomatitis Denture Corpus albicans Yeast Enzyme chemistry Phospholipases Biofilms Agronomy and Crop Science Peptide Hydrolases |
Zdroj: | Mycopathologia. 169:269-278 |
ISSN: | 1573-0832 0301-486X |
Popis: | Several virulence factors in Candida albicans strains such as production of hydrolytic enzymes and biofilm formation on surfaces and cells can contribute to their pathogenicity. For this, control of this opportunistic yeast is one of the factors reducing the nosocomial infection. The aim of this study was to investigate biofilm formation on polystyrene and polymethylmethacrylate and the production of hydrolytic enzymes in Candida albicans strains isolated from the oral cavity of patients suffering from denture stomatitis. All strains were identified by macroscopic, microscopic analysis and the ID 32 C system. Our results showed that 50% of the total strains produced phospholipase. Furthermore, protease activity was detected in seven (35%) strains. All Candida albicans strains were beta haemolytic. All C. albicans strains adhered to polystyrene 96-well microtiter plate at different degrees, and the metabolic activity of C. albicans biofilm formed on polymethylmethacrylate did not differ between tested strains. The atomic force micrographs demonstrated that biofilm of Candida albicans strains was organized in small colonies with budding cells. |
Databáze: | OpenAIRE |
Externí odkaz: |