The HMGA gene family in chordates: evolutionary perspectives from amphioxus
| Autor: | Matteo Bozzo, Paola Ramoino, Guidalberto Manfioletti, Mario Pestarino, Riccardo Sgarra, Robert Vignali, Simona Candiani, Daniela Calzia, Simone Macrì, Thurston C. Lacalli |
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| Přispěvatelé: | Bozzo, M, Macrì, S, Calzia, D, Sgarra, Riccardo, Manfioletti, Guidalberto, Ramoino, P, Lacalli, T, Vignali, R, Pestarino, M, Candiani, S. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Photoreceptors animal structures Amphioxus Development Evodevo HMGA Non-histone chromosomal proteins Pharyngeal skeleton Lamellar Body Evolution Chordates Cellular differentiation Amphioxus Development Evodevo HMGA Non-histone chromosomal proteins Photoreceptors Chordate Evolution Molecular 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission biology.animal amphioxus developmental biology neurobiology Genetics Animals Gene family Neoplastic transformation Amino Acid Sequence Cloning Molecular HMGA Proteins Phylogeny Lancelets biology neurobiology Vertebrate biology.organism_classification 030104 developmental biology Neurula Evolutionary biology Sequence Alignment 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development Genes and Evolution |
| Popis: | High mobility group A proteins of vertebrates, HMGA1 and 2, are chromatin architectural factors involved in development, cell differentiation, and neoplastic transformation. Here, we characterize an amphioxus HMGA gene ortholog and analyze its expression. As a basal chordate, amphioxus is well placed to provide insights into the evolution of the HMGA gene family, particularly in the transition from invertebrates to vertebrates. Our phylogenetic analysis supports the basal position of amphioxus, echinoderm, and hemichordate HMGA sequences to those of vertebrate HMGA1 and HMGA2. Consistent with this, the genomic landscape around amphioxus HMGA shares features with both. Whole mount in situ hybridization shows that amphioxus HMGA mRNA is detectable from neurula stage onwards in both nervous and non-nervous tissues. This correlates with protein expression monitored immunocytochemically using antibodies against human HMGA2 protein, revealing especially high levels of expression in cells of the lamellar body, the amphioxus homolog of the pineal, suggesting that the gene may have, among its many functions, an evolutionarily conserved role in photoreceptor differentiation. |
| Databáze: | OpenAIRE |
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