p53 protein and p21 mRNA levels and caspase-3 activity are altered by zinc status in aortic endothelial cells
| Autor: | Jessica Fanzo, Scott K. Reaves, Kai Y. Lei, Lei Zhu, Libin Cui |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Programmed cell death Physiology chemistry.chemical_element Caspase 3 Zinc Bcl-2-associated X protein Cyclins Proto-Oncogene Proteins Gene expression Humans RNA Messenger Aorta Cells Cultured bcl-2-Associated X Protein Cell Nucleus biology Osmolar Concentration Cell Biology Genes p53 Caspase Inhibitors Molecular biology Neoplasm Proteins Endothelial stem cell Proto-Oncogene Proteins c-bcl-2 chemistry Biochemistry Apoptosis Cell culture Caspases biology.protein Myeloid Cell Leukemia Sequence 1 Protein Endothelium Vascular Tumor Suppressor Protein p53 |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1522-1563 0363-6143 |
| Popis: | The influence of zinc status on the levels of p53, as well as downstream targets of p53 in cell repair and survival, was examined in human aortic endothelial cells (HAECs). A serum-reduced low-zinc medium (ZD) was used to deplete zinc over one passage. Other treatments included zinc-normal control (ZN), zinc-adequate (ZA), and zinc-supplemented (ZS) treatment with 3.0, 16.0, and 32.0 μM zinc, respectively. Cellular zinc levels in the ZD cells were 64% of ZN controls; levels in the ZA cells were not different, but levels in ZS cells were significantly higher (40%) than in ZN cells. No difference in p53 mRNA abundance was detected among all treatments; however, p53 nuclear protein levels were >100% higher in the ZD and ZS cells and almost 200% higher in the ZA cells than in ZN controls. In addition, p21 mRNA abundance, a downstream target of p53 protein, was increased in the ZS cells compared with both the ZN control and ZD cells. In the ZS cells, bax and mcl-1 were also ∼50% higher compared with ZN controls, whereas bcl-2 mRNA was increased compared with ZA cells. Moreover, caspase-3 activity of ZD cells was not different from that of ZN controls but was reduced to 83 and 69% of ZN controls in ZA and ZS cells, respectively. Thus p53 protein and p53 downstream target genes appeared to be modulated by intracellular zinc status in HAECs. |
| Databáze: | OpenAIRE |
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