Platelet-rich plasma in umbilical cord blood reduces neuropathic pain in spinal cord injury by altering the expression of ATP receptors
| Autor: | Atousa Janzadeh, Farinaz Nasirinezhad, Fatemeh Ramezani, Behnaz Rahimi, Zahra Behroozi |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Nervous system Experimental and Cognitive Psychology Umbilical cord Rats Sprague-Dawley Behavioral Neuroscience Threshold of pain medicine Animals Rats Wistar Spinal cord injury Spinal Cord Injuries Platelet-Rich Plasma Receptors Purinergic P2 business.industry Fetal Blood medicine.disease Rats Disease Models Animal Allodynia medicine.anatomical_structure Spinal Cord Hyperalgesia Platelet-rich plasma Anesthesia Neuropathic pain Neuralgia medicine.symptom business |
| Zdroj: | Physiology & Behavior. 228:113186 |
| ISSN: | 0031-9384 |
| Popis: | Neuropathic pain following injury or dysfunction of the peripheral or CNS is one of the most important medical challenges to treat. Humane platelet-rich plasma (HPRP), which is a rich source of growth factors, may be able to treat and reduce pain caused by spinal cord injury (SCI). In this study, the effect of HPRP on neuropathic pain caused by SCI was investigated.Sixty adult male Wistar rats were randomly divided into 6 groups: control, sham, SCI, vehicle (SCI+platelet-poor plasma), SCI+ PRP2day (injection 48 hrs after SCI) and SCI+PRP14day (injection 14 days after SCI). SCI was induced at the T12-T13 level. Behavioral tests were conducted weekly after injury for six weeks. Allodynia and hyperalgesia were assessed using acetone drops, plantar test and von Frey filament. Cavity size and the number of fibroblasts were determined by HE stain, and the expression of mTOR, p-mTOR, PPRP injection showed a higher pain threshold in mechanical allodynia (p0.0001), cold allodynia (p0.0001) and thermal hyperalgesia (p0.0001) than those in the spinal. Animals treated with PRP also reduced cavity size, fibroblast number, p-mTOR/mTOR ratio, and PThe results showed that PRP reduced SCI-induced allodynia and hyperalgesia by regulating ATP signaling. Using HPRP can open a new window in the treatment of pain caused by damage to the nervous system. |
| Databáze: | OpenAIRE |
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