Y chromosome loss and rearrangement in non-small-cell lung cancer
| Autor: | Rob Center, Robyn Lukeis, Vickie Vrazas, O. Margaret Garson |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Lung Neoplasms Euchromatin Heterochromatin Biology Y chromosome Translocation Genetic Carcinoma Non-Small-Cell Lung Y Chromosome Centromere Tumor Cells Cultured medicine Humans Genes Suppressor In Situ Hybridization Fluorescence Genetics Cytogenetics Karyotype Gene rearrangement Molecular biology Oncology Tumor progression Karyotyping Chromosome Deletion |
| Zdroj: | International Journal of Cancer. 55:390-393 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910550309 |
| Popis: | While loss of the Y chromosome from the karyotype of tumor cells has frequently been found in a number of human malignancies of different types, structural alterations are a much less common finding. Prompted by the high frequency of cytogenetic Y chromosome loss found in primary non-small-cell lung cancer (NSCLC), and the fact that NSCLC karyotypes usually contain marker chromosomes of unidentified origin, we have determined the Y chromosome status of 12 NSCLC samples (7 cell lines and 5 primary tumors) at a molecular level. Of the 9 cases which did not have a cytogenetically detectable Y chromosome, 4 were negative for all the Y sequences tested. The other 5, in contrast, retained some Y chromosome sequences. In 1 case (H520), only Yq heterochromatic sequences were detected, whereas in the remaining 4 (L162, L93, L125 and L71) both Yq heterochromatic sequences and Y euchromatic sequences were retained. The region of common overlap for loss of Y euchromatin was Yp distal to the Y centromere. We hypothesize that deletion of Yp sequences may play a role in tumor progression in NSCLC due to loss of a tumor-suppressor gene. |
| Databáze: | OpenAIRE |
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