Transforming growth factor - α activity in effusions: Comparison of radioimmunoassay and radioreceptorassay
| Autor: | Yoshinobu Kamiya, Hiroshi Demura, Toshio Tsushima, Kazuo Shizume, Nagahiko Sakuma, Hitomi Murakami, Eiji Ohmura |
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| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Radioimmunoassay General Biochemistry Genetics and Molecular Biology Radioligand Assay Pleural disease Epidermal growth factor Internal medicine Ascites Biomarkers Tumor medicine Ascitic Fluid Humans General Pharmacology Toxicology and Pharmaceutics Aged business.industry Cancer General Medicine Middle Aged Transforming Growth Factor alpha medicine.disease Pleural Effusion Malignant Pleural Effusion Endocrinology Pleurisy Female medicine.symptom business Quantitative analysis (chemistry) hormones hormone substitutes and hormone antagonists Transforming growth factor |
| Zdroj: | Life Sciences. 52:1381-1386 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(93)90173-z |
| Popis: | Transforming growth factor-alpha (TGF-alpha) in pleural and peritoneal effusions was assayed by homologous radioimmunoassay (RIA) and radioreceptor assay (RRA) using human placental membrane. Effusions were obtained from 24 patients with and 17 patients without cancer. Most of the effusions were found to contain TGF-alpha by RIA and RRA, but immunoreactive epidermal growth factor (EGF) was not detected. Effusions were chromatographed on Bio-Gel P-60 with several peaks of TGF-alpha activity by both RIA and RRA. A discrepancy in the chromatographic pattern of TGF-alpha between RIA and RRa suggested the existence of EGF-like substances capable of binding to EGF receptors which lack immunoreactivity for EGF or TGF-alpha. The TGF-alpha concentration of the acetic acid-extracted malignant effusions assayed by RRA significantly exceeded the value obtained from benign effusions (17.0 +/- 8.7 vs. 9.2 +/- 6.3 ng/ml; mean +/- SD: p0.02). However, the concentrations obtained by RIA did not differ. |
| Databáze: | OpenAIRE |
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