Neuroprotection of microglia conditioned media from apoptotic death induced by staurosporine and glutamate in cultures of rat cerebellar granule cells
| Autor: | Simona Eleuteri, Elisabetta Polazzi, Antonio Contestabile |
|---|---|
| Přispěvatelé: | Eleuteri S., Polazzi E., Contestabile A. |
| Rok vydání: | 2008 |
| Předmět: |
Cerebellum
Glutamic Acid Tetrazolium Salts Apoptosis Cell Count Biology Neuroprotection medicine Animals Staurosporine Enzyme Inhibitors Rats Wistar Cells Cultured Neurons Analysis of Variance Microglia General Neuroscience Neurodegeneration Glutamate receptor medicine.disease Rats Thiazoles medicine.anatomical_structure Animals Newborn nervous system Culture Media Conditioned Neuroglia Neuron Neuroscience medicine.drug |
| Zdroj: | Neuroscience Letters. 448:74-78 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2008.09.033 |
| Popis: | Microglia, the immune cells of the mammalian CNS, have often been indicated as dangerous effector cells for their activation in response to traumatic CNS injuries or immunological stimuli and for their involvement in many chronic neurodegenerative diseases. Recently, several in vitro and in vivo studies have emphasized that microglial activity is essential in promoting neuronal survival. We have tested the efficacy of media directly conditioned by microglia or conditioned by microglia after having been exposed to apoptotic neurons, towards neuroprotection of rat cerebellar granule cells (CGCs) challenged with staurosporine or glutamate. Apoptotic death of CGC caused by staurosporine, as well as by a mild excitotoxic stimulus delivered through sub-chronic glutamate treatment, was significantly counteracted by microglia conditioned media. On the other hand, an acute excitotoxic insult delivered through a short pulse of glutamate exposure in the absence of magnesium and resulting in a mix of apoptotic and necrotic death was only marginally counteracted by microglia conditioned media. The present results extend the available information regarding the neuroprotective role of microglia and support the usefulness of employing the culture approach for perspective identification of neuroprotective factors released by these cells. Furthermore, the use of media previously exposed to apoptotic neurons to elicit the neuroprotective response of microglia, indicate the feasibility to re-create also in the isolated culture conditions, at least some of the elements at the basis of neuron/microglia cross-talk. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect