Placental defects lead to embryonic lethality in mice lacking the Formin and PCP proteins Daam1 and Daam2

Autor: Rieko Ajima, Kristibjorn Orri Gudmundsson, Yuko Komiya, Masa Aki Nakaya, Terry P. Yamaguchi, Raymond Habas, Jonathan R. Keller
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
rho GTP-Binding Proteins
Embryology
Physiology
Placenta
Cellular differentiation
Biochemistry
Mice
Contractile Proteins
0302 clinical medicine
Animal Cells
Pregnancy
Red Blood Cells
Cell polarity
Medicine and Health Sciences
Morphogenesis
Neural Tube Defects
Wnt Signaling Pathway
Cytoskeleton
Mice
Knockout
0303 health sciences
DAAM1
Multidisciplinary
Microfilament Proteins
Wnt signaling pathway
Cell Polarity
Gene Expression Regulation
Developmental
Gene targeting
Cell Differentiation
Body Fluids
Trophoblasts
Cell biology
Actin Cytoskeleton
Blood
Formins
Medicine
Female
Anatomy
Cellular Types
Research Article
Science
Embryonic Development
Biology
03 medical and health sciences
Embryonic morphogenesis
Congenital Disorders
Animals
Birth Defects
Adaptor Proteins
Signal Transducing
030304 developmental biology
Blood Cells
Embryos
Biology and Life Sciences
Proteins
Cell Biology
Actin cytoskeleton
Actins
Placentation
Mice
Inbred C57BL
Cytoskeletal Proteins
Cardiovascular Anatomy
biology.protein
Blood Vessels
Blastocysts
Carrier Proteins
030217 neurology & neurosurgery
Developmental Biology
Zdroj: PLoS ONE, Vol 15, Iss 4, p e0232025 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: The actin cytoskeleton plays a central role in establishing cell polarity and shape during embryonic morphogenesis. Daam1, a member of the Formin family of actin cytoskeleton regulators, is a Dvl2-binding protein that functions in the Wnt/Planar Cell Polarity (PCP) pathway. To examine the role of the Daam proteins in mammalian development, we generated Daam-deficient mice by gene targeting and found that Daam1, but not Daam2, is necessary for fetal survival. Embryonic development of Daam1 mutants was delayed most likely due to functional defects in the labyrinthine layer of the placenta. Examination of Daam2 and Daam1/2 double mutants revealed that Daam1 and Daam2 are functionally redundant during placental development. Of note, neural tube closure defects (NTD), which are observed in several mammalian PCP mutants, are not observed in Wnt5a or Daam1 single mutants, but arise in Daam1;Wnt5a double mutants. These findings demonstrate a unique function for Daam genes in placental development and are consistent with a role for Daam1 in the Wnt/PCP pathway in mammals.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje