IL-10 inhibits vascular smooth muscle cell activation in vitro and in vivo
| Autor: | El Mostafa Mtairag, Walter Gonzalez, Laurent J. Feldman, Mikael Mazighi, Jean-Baptiste Michel, Monique Philippe, Anne Pellé, Dominique Hénin |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Intimal hyperplasia Vascular smooth muscle Physiology medicine.medical_treatment Myocytes Smooth Muscle Gene Expression Biology Muscle Smooth Vascular Catheterization Cell Movement In vivo Physiology (medical) medicine Animals Myocyte Receptors Interleukin-10 Rats Wistar Aorta Cells Cultured Hyperplasia Interleukin-6 Cell growth NF-kappa B Receptors Interleukin medicine.disease Recombinant Proteins Interleukin-10 Rats Cell biology Interleukin 10 Cytokine Immunology Wounds and Injuries I-kappa B Proteins Tunica Intima Cardiology and Cardiovascular Medicine Cell activation Cell Division |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 287:H866-H871 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.00918.2003 |
| Popis: | The anti-inflammatory cytokine IL-10 inhibits intimal hyperplasia after stent implantation via a powerful inactivation of monocytes. We tested the hypothesis that IL-10 may also inhibit vascular smooth muscle cell (SMC) activation via the inhibition of the NF-kappaB/I-kappaB system. The IL-10 receptor was detected in rat SMCs in vitro and in vivo. In LPS-stimulated rat SMCs, 1 ng/ml recombinant murine IL-10 (mIL-10) reduced I-kappaBalpha and I-kappaBbeta degradation, NF-kappaB activation, as well as the expression of the NF-kappaB-dependent gene IL-6 by 32%, 31%, 75%, and 19%, respectively (P0.05 for all). Similar results were obtained in vivo 6 h and 4 days after balloon abrasion of the rat aorta, a model in which intimal hyperplasia results essentially from SMC activation. Moreover, mIL-10 reduced SMC proliferation and migration in vitro (by 60% for both, P0.0001), resulting in reduced SMC proliferation and intimal growth 14 days after balloon abrasion of the rat aorta (by 76% and 75%, respectively; P0.005). In conclusion, mIL-10 has a direct inhibitory effect on SMCs in vitro and in vivo. This effect is mediated in part by NF-kappaB inactivation and may participate in the overall protective effect of IL-10 on postangioplasty restenosis. |
| Databáze: | OpenAIRE |
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