NOVEL EPIGENETIC BIOMARKER DETERMINATION FOR OSCC BY ARRAY-BASED EPIGENOMIC AND TRANSCRIPTOMIC TECHNIQUES
| Autor: | DEMOKAN, Semra, SEN, Sena, ERYILMAZ, Onder, COMERT, Sevde, ULUSAN, Murat, DALAY, Mehmet Nejat |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Volume: 5, Issue: S-1 45-45 Sağlık Bilimlerinde İleri Araştırmalar Dergisi |
| ISSN: | 2651-4060 |
| Popis: | Objectives: Oral squamous cell carcinoma (OSCC) has a high morbidity and mortality rates, but there are no reliable biomarkers to define patients in earlyphases of disease. In our study (TUBITAK-SBAG-114S497), we aimed to investigate the potential epigenetic biomarker candidate genes observedmethylation-dependent expression loss via methylation and expression array methods in OSCC patients.Material and Methods: Methylation and expression profiling in tumor and conjugate-normal tissue samples of 6 OSCC patients were analyzed by“IlluminaHumanMethylation450 chips” and “Illumina iScan”, respectively. Methylation/expression array data were analyzed and interpreted by R(v3.5.1)environment using ChAMP and limma/lumi packages, and then the significant decreased expression changes due to hypermethylation of the candidategene was detected. The selected candidate gene was validated in tumor and matched-normal tissues and body fluids (serum and saliva) of 20 OSCCpatients by QRT-PCR/QMSP methods.Results: According to the array results, it was determined that the expression levels of the candidate gene were decreased due to methylation(DiffScore:13.18826; FoldChange:-1.08345). This candidate gene (unpublished data), which plays an important role in ubiquitin-ligase activity, was foundto be methylated in 45% tumor, 40% matched-normal tissue, 10% serum and 30% saliva samples. 50% of the patients observed methylation in the tumortissue showed the differentially decreased expression levels.Conclusion: It is thought that this candidate gene, whose expression level decreased due to methylation, will be a candidate epigenetic biomarker for theearly diagnosis of the subtypes of OSCC. Further validation of this candidate gene will be needed in the larger OSCC cohorts. |
| Databáze: | OpenAIRE |
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