Cloning of the mouse laminin alpha 4 cDNA. Expression in a subset of endothelium
| Autor: | Astrid Hahn, Rainer Deutzmann, Holger Nöckel, Friederike Pausch, Christine Röder, Michael Frieser, Lydia Sorokin |
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| Rok vydání: | 1997 |
| Předmět: |
DNA
Complementary Endothelium Molecular Sequence Data Alpha (ethology) Gene Expression Biology Biochemistry Mice Laminin medicine Animals Amino Acid Sequence RNA Messenger Cloning Molecular In Situ Hybridization Progesterone Differential display Base Sequence cDNA library Blotting Northern Molecular biology Laminin alpha 4 Endothelial stem cell medicine.anatomical_structure cardiovascular system biology.protein Cytokines Cattle Endothelium Vascular Alpha chain |
| Zdroj: | European journal of biochemistry. 246(3) |
| ISSN: | 0014-2956 |
| Popis: | Endothelial cells express a 400-kDa or 240-kDa laminin alpha chain, depending on their tissue of origin or physiological state [1, 2]. Using differential display and subsequent screening of a mouse endothelial cell cDNA library we here identify the gene coding for the 240-kDa laminin chain as the laminin alpha 4 gene. The complete mouse laminin alpha 4 cDNA sequence is reported and compared with other laminin alpha chains. In situ hybridization of embryonic and new born mouse tissues revealed expression of laminin alpha 4 mRNA in a subset of endothelium, in particular aortic endothelium, endocardium and endothelium of blood vessels in the skin and in the brain. Strong laminin alpha 4 expression by aortic endothelia was confirmed by data obtained from cultured bovine aortic endothelial cells (BAEC). Isolation of laminin from BAEC conditioned medium revealed a Y-shaped molecule in rotary shadowing. Subsequent sequencing of BAEC laminin resulted in laminin alpha 4, beta 1 and gamma 1 amino acid sequences, confirming that laminin alpha 4 is one of the major laminin alpha chains expressed by aortic endothelium not only in the mouse. In addition, strong laminin alpha 4 mRNA expression occurred in peripheral nerves, cardiac muscle, fat, the dermis of the skin and lung stroma of mouse tissues. The data demonstrate a cytokine and progesterone-regulated differential expression of laminin alpha 4 mRNA in mouse endothelium, suggesting a distinct functional role for this laminin chain in endothelium. |
| Databáze: | OpenAIRE |
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