Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine
| Autor: | Paolo Cinà, Salvatrice Rigogliuso, Monica Salamone, Simona Campora, Aldo Nicosia, Giulio Ghersi, Carmelo Bruno, Marcello Tagliavia |
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| Přispěvatelé: | Salamone M., Rigogliuso S., Nicosia A., Tagliavia M., Campora S., Cina P., Bruno C., Ghersi G. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Biology
Regenerative Medicine Regenerative medicine Article Chondrocyte 03 medical and health sciences Chondrocytes 0302 clinical medicine Tissue engineering medicine Animals Humans cell transplantation lcsh:QH301-705.5 030304 developmental biology 0303 health sciences nasal chondrocytes Tissue Engineering gene expression profiles Cartilage Neural crest Cell Differentiation General Medicine tissue dissociation In vitro 3. Good health Cell biology Transplantation medicine.anatomical_structure lcsh:Biology (General) Neural Crest collagenases Collagenase Cattle 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Cells Volume 9 Issue 4 Cells, Vol 9, Iss 962, p 962 (2020) |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells9040962 |
| Popis: | Chondrocyte transplantation has been successfully tested and proposed as a clinical procedure aiming to repair articular cartilage defects. However, the isolation of chondrocytes and the optimization of the enzymatic digestion process, as well as their successful in vitro expansion, remain the main challenges in cartilage tissue engineering. In order to address these issues, we investigated the performance of recombinant collagenases in tissue dissociation assays with the aim of isolating chondrocytes from bovine nasal cartilage in order to establish the optimal enzyme blend to ensure the best outcomes of the overall procedure. We show, for the first time, that collagenase H activity alone is required for effective cartilage digestion, resulting in an improvement in the yield of viable cells. The extracted chondrocytes proved able to grow and activate differentiation/dedifferentiation programs, as assessed by morphological and gene expression analyses. |
| Databáze: | OpenAIRE |
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