Short-term hypoxia and vasa recta function in kidney slices
| Autor: | Inggrid Christine Wennysia, Pontus B. Persson, Andreas Patzak, Tamara Margit Jutta Pahlitzsch, Stefanie Dietze, Marion Ludwig, Diana Braun |
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| Rok vydání: | 2017 |
| Předmět: |
Male
medicine.medical_specialty Physiology 030232 urology & nephrology 030204 cardiovascular system & hematology Kidney Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Carbogen Physiology (medical) Internal medicine medicine Animals Medulla Kidney Medulla Renal ischemia Chemistry Vasa recta Hematology Hypoxia (medical) medicine.disease Cell Hypoxia Rats Perfusion Endocrinology medicine.anatomical_structure medicine.symptom Cardiology and Cardiovascular Medicine Reperfusion injury |
| Zdroj: | Clinical Hemorheology and Microcirculation. 67:475-484 |
| ISSN: | 1875-8622 1386-0291 |
| Popis: | Background Descending vasa recta (DVR) supply the inner part of outer renal medulla an area at risk for hypoxic damages. Objective We hypothesize increased vasoreactivity after hypoxia/re-oxygenation (H/R) in DVR, which might contribute to the reduced medullary perfusion after an ischemic event. Methods Live kidney slices (200μm) from SD rats were used for functional experiments. TUNEL assay and H&E staining were used to estimate slice viability. Kidney slices were treated with carbogen or hypoxia (1% O2) for 60 or 90 min and vasoreactivity to Ang II (10-7 M) was recorded by DIC microscopy after re-oxygenation with carbogen. Expression of NOS and NADPH enzymes mRNA were determined in iron-perfusion isolated VR. Results Percentage of apoptotic cells increased in control and H/R after 90 min in the medulla. Ang II- induced constriction of DVR was reduced after 90 min in control (compared to 60 min), but not after H/R. NOS enzymes mRNA expression levels decreased over 90 min hypoxia. Conclusions Increased reactivity of DVR to Ang II after H/R compared to control (90 min) suggest a role of DVR in renal ischemia/reperfusion injury. |
| Databáze: | OpenAIRE |
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