Preservability of bull spermatozoa in Tris-egg yolk extender enriched with different concentrations of butylated hydroxytoluene
Autor: | Gamal A. El-Sisy, Walid S. El-Nattat, Reda I. El-Sheshtawy |
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Rok vydání: | 2017 |
Předmět: |
BHT
endocrine system food.ingredient lcsh:Medicine Semen Plant Science Cryopreservation law.invention chemistry.chemical_compound fluids and secretions food law Yolk Butylated hydroxytoluene Acrosome Sperm motility Chromatography General Veterinary urogenital system Chemistry lcsh:R Extender Obstetrics and Gynecology Sperm Preservation Reproductive Medicine Cattle Animal Science and Zoology |
Zdroj: | Asian Pacific Journal of Reproduction, Vol 6, Iss 2, Pp 77-80 (2017) |
ISSN: | 2305-0519 2305-0500 |
DOI: | 10.12980/apjr.6.20170205 |
Popis: | Objective: To explore the effect of BHT on cattle spermatozoa during cooling and cryopreservation.Methods: Pooled bull semen were diluted by Tris-Citrate-Fructose egg yolk (TCFY) diluent considered as control (0 BHT) and different concentrations of BHT (1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 mM were prepared in ethanol in prewarmed (37 °C) test tubes. The ethanol was allowed to evaporate so that, a thin crystallized layer of BHT was deposited on the inner surface of the tubes. Then extended semen was added into the tubes and incubated at 37 °C for 5 min to allow uptake of BHT by spermatozoa. The tubes were cooled slowly (approximately for 2 h) up to 5 °C and equilibrated for 4 h. After equilibration, semen freezing process was carried out. Extended semen was subjected to evaluation (motility, alive sperm, intact sperm membrane (HOST) % and acrosome integrity) in both cooled and cryopreserved semen.Results: The result revealed that sperm motility of post-cooled spermatozoa improved (P |
Databáze: | OpenAIRE |
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