A cross-sectional screening by next-generation sequencing reveals Rickettsia, Coxiella, Francisella, Borrelia, Babesia, Theileria and Hemolivia species in ticks from Anatolia
Autor: | Olcay Hekimoglu, Koray Ergünay, Peter Hagedorn, Annika Brinkmann, Ender Dinçer, Andreas Nitsche |
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Přispěvatelé: | Biyoloji |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Turkey Rhipicephalus sanguineus Hyalomma marginatum 030231 tropical medicine Theilera Zoology Babesia Tick lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Ticks Coxiella Species Specificity Theileria Next generation sequencing parasitic diseases Animals Humans lcsh:RC109-216 ddc:610 Rickettsia Francisella Arthropods biology Bacteria Research Borrelia Arthropod Vectors biology.organism_classification Haemaphysalis bacterial infections and mycoses Rhipicephalus 030104 developmental biology Infectious Diseases Cross-Sectional Studies Hemolivia Parasitology Hyalomma 610 Medizin und Gesundheit Nucleic Acid Amplification Techniques |
Zdroj: | Parasites & Vectors, Vol 12, Iss 1, Pp 1-13 (2019) Parasites & Vectors |
ISSN: | 1756-3305 |
Popis: | Background Ticks participate as arthropod vectors in the transmission of pathogenic microorganisms to humans. Several tick-borne infections have reemerged, along with newly described agents of unexplored pathogenicity. In an attempt to expand current information on tick-associated bacteria and protozoans, we performed a cross-sectional screening of ticks, using next-generation sequencing. Ticks seeking hosts and infesting domestic animals were collected in four provinces across the Aegean, Mediterranean and Central Anatolia regions of Turkey and analyzed by commonly used procedures and platforms. Results Two hundred and eighty ticks comprising 10 species were evaluated in 40 pools. Contigs from tick-associated microorganisms were detected in 22 (55%) questing and 4 feeding (10%) tick pools, with multiple microorganisms identified in 12 pools. Rickettsia 16S ribosomal RNA gene, gltA, sca1 and ompA sequences were present in 7 pools (17.5%), comprising feeding Haemaphysalis parva and questing/hunting Rhipicephalus bursa, Rhipicephalus sanguineus (sensu lato) and Hyalomma marginatum specimens. A near-complete genome and conjugative plasmid of a Rickettsia hoogstraalii strain could be characterized in questing Ha. parva. Coxiella-like endosymbionts were identified in pools of questing (12/40) as well as feeding (4/40) ticks of the genera Rhipicephalus, Haemaphysalis and Hyalomma. Francisella-like endosymbionts were also detected in 22.5% (9/40) of the pools that comprise hunting Hyalomma ticks in 8 pools. Coxiella-like and Francisella-like endosymbionts formed phylogenetically distinct clusters associated with their tick hosts. Borrelia turcica was characterized in 5% (2/40) of the pools, comprising hunting Hyalomma aegyptium ticks. Co-infection of Coxiella-like endosymbiont and Babesia was noted in a questing R. sanguineus (s.l.) specimen. Furthermore, protozoan 18S rRNA gene sequences were detected in 4 pools of questing/hunting ticks (10%) and identified as Babesia ovis, Hemolivia mauritanica, Babesia and Theileria spp. Conclusions Our metagenomic approach enabled identification of diverse pathogenic and non-pathogenic microorganisms in questing and feeding ticks in Anatolia. Electronic supplementary material The online version of this article (10.1186/s13071-018-3277-7) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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