Electrical Guidance of Human Stem Cells in the Rat Brain
| Autor: | Min Zhao, Jing Liu, Bruce G. Lyeth, Lei Zhang, Jun Feng Feng, Ji Yao Jiang, Michael J. Russell, Jan A. Nolta |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Stimulation Regenerative Medicine Biochemistry Green fluorescent protein electrotaxis Rats Sprague-Dawley Electricity Neural Stem Cells Stem Cell Research - Nonembryonic - Human galvanotaxis Cell Movement lcsh:QH301-705.5 electric stimulation Electric stimulation Cancer in vivo migration lcsh:R5-920 Brain Anatomy Equipment Design Neural stem cell 3. Good health electric field Cell Tracking Neurological Stem Cell Research - Nonembryonic - Non-Human Stem cell lcsh:Medicine (General) neuroblasts directional cell migration 1.1 Normal biological development and functioning Neurogenesis Clinical Sciences Green Fluorescent Proteins Biology Article Cell Line 03 medical and health sciences Rare Diseases Neuroblast Genetics Animals Humans Stem Cell Research - Embryonic - Human Transplantation 5.2 Cellular and gene therapies Neurosciences Cell Biology Stem Cell Research Rat brain Electric Stimulation Brain Disorders Rats Brain Cancer 030104 developmental biology human neural stem cells lcsh:Biology (General) brain in vivo Sprague-Dawley Biochemistry and Cell Biology Neuroscience Developmental Biology |
| Zdroj: | Stem Cell Reports Stem cell reports, vol 9, iss 1 Stem Cell Reports, Vol 9, Iss 1, Pp 177-189 (2017) Feng, JF; Liu, J; Zhang, L; Jiang, JY; Russell, M; Lyeth, BG; et al.(2017). Electrical Guidance of Human Stem Cells in the Rat Brain. Stem Cell Reports, 9(1), 177-189. doi: 10.1016/j.stemcr.2017.05.035. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/78t424mv |
| ISSN: | 2213-6711 |
| Popis: | Summary Limited migration of neural stem cells in adult brain is a roadblock for the use of stem cell therapies to treat brain diseases and injuries. Here, we report a strategy that mobilizes and guides migration of stem cells in the brain in vivo. We developed a safe stimulation paradigm to deliver directional currents in the brain. Tracking cells expressing GFP demonstrated electrical mobilization and guidance of migration of human neural stem cells, even against co-existing intrinsic cues in the rostral migration stream. Transplanted cells were observed at 3 weeks and 4 months after stimulation in areas guided by the stimulation currents, and with indications of differentiation. Electrical stimulation thus may provide a potential approach to facilitate brain stem cell therapies. Graphical Abstract Highlights • Developed a technology and device delivering electric current to the brain in vivo • Achieved stable delivery of currents to brain with monitoring and safety concerns • Exhibited effective guidance of migration of transplanted human NSCs in live brain • Demonstrated enhanced motility, survival, and differentiation of the guided hNSCs In this article, Zhao and colleagues report a novel technology delivering directional electric currents which mobilizes and guides human neural stem cells through the brain in vivo, demonstrating an effective and safe approach to facilitate stem cell therapy, with significant implications for a wide range of brain diseases. |
| Databáze: | OpenAIRE |
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