A prime-boost immunisation regimen using recombinant BCG and Pr55(gag) virus-like particle vaccines based on HIV type 1 subtype C successfully elicits Gag-specific responses in baboons
| Autor: | James Maclean, Carolyn Williamson, Enid G. Shephard, Edward P. Rybicki, William R. Bourn, Ann E. Meyers, Gerald K. Chege, Robin Thomas, Clive M. Gray, Anna-Lise Williamson |
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| Rok vydání: | 2008 |
| Předmět: |
viruses
Genetic Vectors Immunization Secondary Biology CD8-Positive T-Lymphocytes HIV Antibodies Recombinant virus complex mixtures Virus Epitope Interferon-gamma South Africa Immune system Virus-like particle Animals Protein Precursors AIDS Vaccines Vaccines Synthetic General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health virus diseases Group-specific antigen Virology Mycobacterium bovis Bacterial vaccine Vaccines Virosome Infectious Diseases Immunology HIV-1 Molecular Medicine BCG vaccine Papio |
| Zdroj: | Vaccine. 27(35) |
| ISSN: | 1873-2518 |
| Popis: | Mycobacterium bovis BCG is considered an attractive live bacterial vaccine vector. In this study, we investigated the immune response of baboons to a primary vaccination with recombinant BCG (rBCG) constructs expressing the gag gene from a South African HIV-1 subtype C isolate, and a boost with HIV-1 subtype C Pr55(gag) virus-like particles (Gag VLPs). Using an interferon enzyme-linked immunospot assay, we show that although these rBCG induced only a weak or an undetectable HIV-1 Gag-specific response on their own, they efficiently primed for a Gag VLP boost, which strengthened and broadened the immune responses. These responses were predominantly CD8+ T cell-mediated and recognised similar epitopes as those targeted by humans with early HIV-1 subtype C infection. In addition, a Gag-specific humoral response was elicited. These data support the development of HIV-1 vaccines based on rBCG and Pr55(gag) VLPs. |
| Databáze: | OpenAIRE |
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