Event-specific quantitative polymerase chain reaction methods for detection of double-herbicide-resistant genetically modified corn MON 87419 based on the 3′-junction of the insertion site
| Autor: | Li Congcong, Long Likun, Yan Wei, Liu Na, Dong Liming, Li Feiwu, Xing Zhenjuan |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Biology Polymerase Chain Reaction Zea mays 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry law.invention 03 medical and health sciences chemistry.chemical_compound law Dicamba Molecular Biology Polymerase chain reaction Genetically modified maize 010401 analytical chemistry Organic Chemistry General Medicine Amplicon Plants Genetically Modified Molecular biology 0104 chemical sciences Genetically modified organism 030104 developmental biology Real-time polymerase chain reaction chemistry Primer (molecular biology) DNA Herbicide Resistance Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 85:1468-1475 |
| ISSN: | 1347-6947 |
| DOI: | 10.1093/bbb/zbab040 |
| Popis: | MON 87419 was one of the new transgenic corn events developed in US with the trait of herbicide resistance to both dicamba and glyphosate. To monitor unintended release of genetically modified organism in the future, as well as to meet GM-labeling requirements, it is requisite to develop a reliable method for the detection and quantification of MON 87419, an event-specific primer pair was designed to amplify the 3′-junction site between the endogenous genome sequence and the transferred DNA of GM event MON 87419, amplicons of desired size were produced by qualitative polymerase chain reaction (PCR) assay. For the validation of this quantitative method, the mixed samples containing 10%, 1%, and 0.1% MON 87419 ingredient were quantified. The precisions were expressed as relative standard deviations, deviated by 7.87%, 12.94%, and 19.98%, respectively. These results clearly demonstrate that the PCR methods we developed herein can be used for event-specific quantitative testing of the double-herbicide-resistant corn MON 87419. |
| Databáze: | OpenAIRE |
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