Protective effects of methane-rich saline on diabetic retinopathy via anti-inflammation in a streptozotocin-induced diabetic rat model
| Autor: | Xuejun Sun, Fangzhou Xia, Ruobing Wang, Zeli Chen, Jiangchun Wu, Qinglei Sun, Lin Liu, Zhouheng Ye |
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| Rok vydání: | 2015 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Biophysics Blood–retinal barrier Sodium Chloride Biochemistry Streptozocin Diabetes Mellitus Experimental Rats Sprague-Dawley chemistry.chemical_compound Diabetes mellitus Internal medicine Blood-Retinal Barrier medicine Animals Molecular Biology Evans Blue Diabetic Retinopathy Glial fibrillary acidic protein biology business.industry Body Weight Retinal Cell Biology Diabetic retinopathy medicine.disease Streptozotocin Rats Vascular endothelial growth factor medicine.anatomical_structure Endocrinology chemistry biology.protein business Methane medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 466:155-161 |
| ISSN: | 0006-291X |
| Popis: | As the commonest complication of diabetes mellitus (DM), diabetic retinopathy (DR) is a neuro-vascular disease with chronic inflammatory. Methane could exert potential therapeutic interest in inflammatory pathologies in previous studies. Our study aims to evaluate the protective effects of methane-rich saline on DR and investigate the potential role of related MicroRNA (miRNA) in diabetic rats. Streptozotocin-induced diabetic Sprague-Dawley rats were injected intraperitoneally with methane-rich or normal saline (5 ml/kg) daily for eight weeks. Morphology changes and blood-retinal barrier (BRB) permeability were assessed by hematoxylin eosin staining and Evans blue leakage. Retinal inflammatory cytokines levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL1-β) were evaluated by immunohistochemistry. Retinal protein expressions of glial fibrillary acidic protein (GFAP) and vascular endothelial growth factor (VEGF) were determined by western blotting. Retinal miRNA expressions were examined by miRNA-specific microarray, verified by quantitative RT-PCR and predicted by GO enrichment and KEGG pathway analysis. There was no significant changes in blood glucose level and body weight of diabetic rats with methane-rich or normal saline treatment, but the decreased retinal thickness, retinal ganglial cell loss and BRB breakdown were all significantly suppressed by methane treatment. DM-induced retinal overexpressions of TNF-α, IL-1β, GFAP and VEGF were also significantly ameliorated. Moreover, the methane treatment significantly up-regulated retinal levels of miR-192-5p (related to apoptosis and tyrosine kinase signaling pathway) and miR-335 (related to proliferation, oxidative stress and leukocyte). Methane exerts protective effect on DR via anti-inflammation, which may be related to the regulatory mechanism of miRNAs. |
| Databáze: | OpenAIRE |
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