Label-Free Measurements of Molecular Transport across Liposome Membranes using Evanescent-Wave Sensing
| Autor: | Magnus Brändén, Sara Dahlin, Fredrik Höök |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Sucrose
Liposome Chemistry Lipid Bilayers Surface plasmon technology industry and agriculture Analytical chemistry Resonance Biological Transport Biosensing Techniques Microfluidic Analytical Techniques Surface Plasmon Resonance Melitten Permeability Atomic and Molecular Physics and Optics Melittin Kinetics chemistry.chemical_compound Membrane Liposomes Biophysics Physical and Theoretical Chemistry Surface plasmon resonance Lipid bilayer Refractive index |
| Zdroj: | ChemPhysChem. 9:2480-2485 |
| ISSN: | 1439-7641 1439-4235 |
| DOI: | 10.1002/cphc.200800614 |
| Popis: | Pucker up! A novel approach enabling direct measurements of biomolecular transfer across lipid bilayer membranes, using surface plasmon resonance, is demonstrated. The figure shows the transfer of sucrose (S) through melittin pores formed in surface-attached liposomes. By measuring the shift in refractive index in the volume enclosed by the liposomes, the sucrose transfer can be time-resolved and quantified. |
| Databáze: | OpenAIRE |
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