Mass spectrometric study of peptides secreted by the skin glands of the brown frog Rana arvalis from the Moscow region
| Autor: | Konstantin A. Artemenko, T. Yu. Samgina, Roman A. Zubarev, S. V. Ogourtsov, Vladimir Gorshkov, Albert T. Lebedev |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Spectrometry Mass Electrospray Ionization Ranidae Molecular Sequence Data Rana arvalis Cystine Peptide High-performance liquid chromatography Fourier transform ion cyclotron resonance Amphibian Proteins Analytical Chemistry Russia chemistry.chemical_compound Anti-Infective Agents Animals Amino Acid Sequence Derivatization Peptide sequence Spectroscopy Chromatography High Pressure Liquid Skin chemistry.chemical_classification Chromatography biology Organic Chemistry biology.organism_classification Rana ridibunda chemistry Female Peptides Oxidation-Reduction |
| Zdroj: | Rapid communications in mass spectrometry : RCM. 23(9) |
| ISSN: | 0951-4198 |
| Popis: | A high-performance liquid chromatography nano-electrospray ionization Fourier transform mass spectrometry (HPLC/nanoESI-FTMS) approach involving recording of collision-activated dissociation (CAD) and electron-capture dissociation (ECD) spectra of an intact sample and two its modifications after performic oxidation and reduction followed by carboxamidomethylation helps to establish peptide profiles in the crude secretion of frog species at mid-throughput level, including de novo sequencing. The proposed derivatization procedures allow increasing of the general sequence coverage in the backbone, providing complementary information and, what is more important, reveal the amino acid sequence in the cystine ring ('rana box'). Thus purely mass spectrometric efficient sequencing becomes possible for longer than usual proteolytic peptides. Seventeen peptides belonging to four known families were identified in the secretion of the European brown frog Rana arvalis inhabiting the Moscow region in Russia. Ranatuerins, considered previously a unique feature of the North American species, as well as a new melittin-related peptide, are worth special mention. The developed approach was previously successfully used for the identification of peptides in the skin secretion of the Caucasian green frog Rana ridibunda. |
| Databáze: | OpenAIRE |
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