p38 mitogen activated protein kinase controls two successive-steps during the early mesodermal commitment of embryonic stem cells.: P38 CONTROLS THE MESODERMAL COMMITMENT OF ES CELLS
| Autor: | Yasmine Hadjal, Ola Hadadeh, Didier Nègre, Valérie M. Renault, Emilie Barruet, Irène Juhan-Vague, Roselyne Tournaire, Bernard Binétruy, Denis Bernot, Marie-Christine Alessi, Franck Peiretti |
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| Přispěvatelé: | Fibrinolyse et Pathologie Vasculaire, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Stress Cellulaire, IFR128, Virologie humaine, École normale supérieure de Lyon (ENS de Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by a grant from the Association Française contre les Myopathies. E. B. was supported by fellowships from the Groupe d'Eude sur l'Hémostase et la Thrombose and from the Fondation pour la Recherche Médicale O. H. was supported by a fellowship from the Association Française contre les Myopathies., École normale supérieure - Lyon (ENS Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), TOURNAIRE, ROSELYNE |
| Rok vydání: | 2010 |
| Předmět: |
Fetal Proteins
Cellular differentiation Cell MESH: Myocytes Cardiac Fluorescent Antibody Technique MESH: Flow Cytometry [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Muscle Development MESH: Mitogen-Activated Protein Kinase 14 Mesoderm Mitogen-Activated Protein Kinase 14 Gene Knockout Techniques Mice [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Myocyte MESH: Animals Myocytes Cardiac MESH: Embryonic Stem Cells MESH: Fluorescent Antibody Technique [SDV.BDD]Life Sciences [q-bio]/Development Biology Cells Cultured MESH: Gene Knockout Techniques Regulation of gene expression MESH: Mesoderm MESH: Fetal Proteins Neurogenesis Imidazoles Cell Differentiation Hematology Flow Cytometry MESH: Gene Expression Regulation Cell biology medicine.anatomical_structure MESH: Muscle Development MESH: Imidazoles MESH: Cells Cultured MESH: Cell Differentiation Brachyury MESH: T-Box Domain Proteins Blotting Western [SDV.CAN]Life Sciences [q-bio]/Cancer Biology [SDV.CAN] Life Sciences [q-bio]/Cancer [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] medicine MESH: Blotting Western Animals MESH: Mice Embryonic Stem Cells Skeletal muscle Cell Biology Embryonic stem cell Gene Expression Regulation Immunology T-Box Domain Proteins Developmental Biology |
| Zdroj: | Stem Cells and Development Stem Cells and Development, 2011, 20 (7), pp.1233-1246. ⟨10.1089/scd.2010.0213⟩ Stem Cells and Development, 2011, 20 (7), pp.1233-46. ⟨10.1089/scd.2010.0213⟩ |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2010.0213⟩ |
| Popis: | International audience; Embryonic stem (ES) cells differentiate in vitro into all cell lineages. We previously found that the p38 mitogen activated kinase (p38MAPK) pathway controls the commitment of ES cells toward either cardiomyogenesis (p38 on) or neurogenesis (p38 off ). In this study, we show that p38α knock-out ES cells do not differentiate into cardiac, endothelial, smooth muscle, and skeletal muscle lineages. Reexpression of p38MAPK in these cells partially rescues their mesodermal differentiation defects and corrects the high level of spontaneous neurogenesis of knock-out cells. Wild-type ES cells were treated with a p38MAPK-specific inhibitor during the differentiation process. These experiments allowed us to identify 2 early independent successive p38MAPK functions in the formation of mesodermal lineages. Further, the first one correlates with the regulation of the expression of Brachyury, an essential mesodermal-specific transcription factor, by p38MAPK. In conclusion, by genetic and biochemical approaches, we demonstrate that p38MAPK activity is essential for the commitment of ES cell into cardiac, endothelial, smooth muscle, and skeletal muscle mesodermal lineages. |
| Databáze: | OpenAIRE |
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