Chlamydia pneumoniae: defining the clinical spectrum of infection requires precise laboratory diagnosis
| Autor: | N F Lightfoot, S J Bourke |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine medicine.medical_specialty Adolescent Coronary Artery Disease medicine.disease_cause Polymerase Chain Reaction Gastroenterology Asymptomatic Serology Immunoenzyme Techniques Mice Internal medicine Animals Humans Medicine Child Respiratory Tract Infections Aged Chlamydia medicine.diagnostic_test Respiratory tract infections biology business.industry Gold standard (test) Chlamydia Infections Chlamydophila pneumoniae medicine.disease Immunoassay Immunology biology.protein Female medicine.symptom Antibody business Research Article |
| Zdroj: | Thorax. 50:S43-S48 |
| ISSN: | 0040-6376 |
| DOI: | 10.1136/thx.50.suppl_1.s43 |
| Popis: | To assess the utility of PCR-enzyme immunoassay (EIA) for diagnosis of acute infection with C pneumoniae, we compared tissue culture, PCR-EIA, direct fluorescent-antibody (DFA) stain, and serology in studies with 56 patients with respiratory symptoms and 80 asymptomatic persons. Thirty five patients were positive by either culture or PCR-EIA, and 101 were negative by both assays. Thirty specimens from symptomatic patients and one from an asymptomatic patient were culture positive ; 23 of these were also PCR-EIA positive. Of the eight culture-positive, PCR-EIA-negative specimens, five were DFA negative and three were DFA positive. Four additional specimens were culture negative and PCR-EIA positive ; of these, three were DFA positive and one was DFA negative. When we used culture- and/or DFA-positive results as a reference or gold standard, the sensitivity and specificity of PCR were 76.5 and 99.0% respectively. When we used PCR- and/or DFA-positive results as the reference, the sensitivity of culture was 87.5%. On the basis of single acute serum specimens, only 8 of these 35 patients had diagnostic antibody titres. Of the asymptomatic patients, 75% had immunoglobulin G or immunoglobulin M antibody to C pneumoniae ; 15 (18.8%) of these had antibody levels considered to be diagnostic of acute infection. This multicentre study indicates that culture and/or PCR-EIA is more reliable for prompt diagnosis of C pneumoniae infection than single-point serology alone. (J Clin Microbiol 1994 ;32 :903-5) |
| Databáze: | OpenAIRE |
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