DNA lesions that block DNA replication are responsible for the dnaA induction caused by DNA damage
| Autor: | Walter Messer, Wolf-Rainer Jueterbock, Ariel Quiñones |
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| Rok vydání: | 1991 |
| Předmět: |
DNA Replication
DNA Bacterial Methylnitronitrosoguanidine Ultraviolet Rays DNA damage Immunoblotting genetic processes Biology Pre-replication complex Bacterial Proteins Escherichia coli Genetics Promoter Regions Genetic SOS Response Genetics Molecular Biology dnaB helicase Circular bacterial chromosome Single-Strand Specific DNA and RNA Endonucleases DNA replication Gene Expression Regulation Bacterial beta-Galactosidase Molecular biology DnaA DNA-Binding Proteins Kinetics DNA Replication Inhibition Prokaryotic DNA replication health occupations bacteria DNA Damage |
| Zdroj: | Molecular and General Genetics MGG. 231:81-87 |
| ISSN: | 1432-1874 0026-8925 |
| Popis: | The initiation protein DnaA of Escherichia coli regulates its own expression autogenously by binding to a 9 bp consensus sequence, the dnaA box, between the promoters dnaAP1 and dnaAP2. In this study, we analysed dnaA regulation in relation to DNA damage and found dnaA expression to be inducible by DNA lesions that inhibit DNA replication. On the other hand, coding DNA lesions were not able to induce dnaA expression. These results suggest that an additional regulatory mechanism is involved in dnaA gene expression and that DnaA protein may play a role in cellular responses to DNA damage. Furthermore, they strongly suggest that in response to DNA replication inhibition by DNA damage, and enhanced (re)initiation capacity is induced by oriC. |
| Databáze: | OpenAIRE |
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