N-glycan profiling of papillary thyroid carcinoma tissues by MALDI-TOF-MS
| Autor: | Yucel Kadioglu, Mehmet Yaman, Mevlut Albayrak, Hacı Mehmet Kayili, Bekir Salih, Ömer Koçak |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male endocrine system Glycan Glycosylation endocrine system diseases Biophysics 01 natural sciences Biochemistry Papillary thyroid cancer Glycomics Thyroid carcinoma 03 medical and health sciences chemistry.chemical_compound Polysaccharides medicine Humans Molecular Biology Thyroid cancer 030304 developmental biology 0303 health sciences biology Esterification 010401 analytical chemistry Thyroid Cell Biology Middle Aged medicine.disease 0104 chemical sciences carbohydrates (lipids) Matrix-assisted laser desorption/ionization medicine.anatomical_structure chemistry ROC Curve Thyroid Cancer Papillary Case-Control Studies Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Cancer research Female |
| Zdroj: | Analytical biochemistry. 584 |
| ISSN: | 1096-0309 |
| Popis: | Papillary thyroid carcinoma (PTC) is a type of thyroid cancer whose incidence rate has increased recently all over the world. Glycosylation is a crucial post-translational modification (PTM) for the regulation of thyroid hormone synthesis in thyroid glands. However, our knowledge regarding the N-glycosylation change in PTC is limited. To the best of our knowledge, this is the first study to profile glycans in PTC tissues by mass spectrometry. Herein, we have analyzed the N-glycans of formalin-fixed paraffin-embedded (FFPE) tissues of patients diagnosed with PTC in a matched case-control study. Using MALDI-TOF(/TOF)-MS, 35 enzymatically released N-glycan compositions were characterized. The statistical analyses showed significant differences including six N-glycan compositions (p 0.001) between patients and controls. It was determined that four of them (H5N4E1, H5N4F1E1, H5N4F1L1E1 and H5N4F1E2, E: α2,6-linked sialic acid; L: α2,3-linked sialic acid) were up-regulated in PTC tissues, whereas two N-glycans (H8N2 and H9N2) found to be down-regulated. Besides, a significant difference was found in six different N-glycan traits. Variants of PTC (follicular, classical, hurtle cell) were also studied to define specific N-glycan change for each variant. |
| Databáze: | OpenAIRE |
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