Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports K+-ion across biological membranes
| Autor: | Sandipan Chakraborty, Biman Jana, Manish Debnath, Ritapa Chaudhuri, Jyotirmayee Dash, Y. Pavan Kumar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Science
Lipid Bilayers Ionophore General Physics and Astronomy 02 engineering and technology CHO Cells Molecular Dynamics Simulation 010402 general chemistry G-quadruplex 01 natural sciences General Biochemistry Genetics and Molecular Biology Article chemistry.chemical_compound Cricetulus Animals Humans Lipid bilayer lcsh:Science Ion transporter Multidisciplinary Ion Transport Molecular Structure Biological membrane Nucleosides General Chemistry 021001 nanoscience & nanotechnology Chemical biology 0104 chemical sciences G-Quadruplexes Chemistry Membrane Spectrometry Fluorescence chemistry Membrane protein Potassium Ionophores Biophysics lcsh:Q 0210 nano-technology K562 Cells DNA |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | The selective transport of ions across cell membranes, controlled by membrane proteins, is critical for a living organism. DNA-based systems have emerged as promising artificial ion transporters. However, the development of stable and selective artificial ion transporters remains a formidable task. We herein delineate the construction of an artificial ionophore using a telomeric DNA G-quadruplex (h-TELO) and a lipophilic guanosine (MG). MG stabilizes h-TELO by non-covalent interactions and, along with the lipophilic side chain, promotes the insertion of h-TELO within the hydrophobic lipid membrane. Fluorescence assays, electrophysiology measurements and molecular dynamics simulations reveal that MG/h-TELO preferentially transports K+-ions in a stimuli-responsive manner. The preferential K+-ion transport is presumably due to conformational changes of the ionophore in response to different ions. Moreover, the ionophore transports K+-ions across CHO and K-562 cell membranes. This study may serve as a design principle to generate selective DNA-based artificial transporters for therapeutic applications. DNA based ionophores can mediate efficient ion transport and are crucial to understand the biological role of their natural counterparts. Here authors engineer a telomeric DNA G-quadruplex based ionophore which transports K+ in a stimuli responsive manner. |
| Databáze: | OpenAIRE |
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