Regulation by protein kinase-C of putative P-type Ca channels expressed in Xenopus oocytes from cerebellar mRNA
| Autor: | Franck Fournier, Gérard Brûlé, Emmanuel Bourinet, Carole Vilbert, Fabrice Matifat, Gilles Charpentier, D. Marlot, Pierre Navarre, Pierre Charnet |
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| Rok vydání: | 1993 |
| Předmět: |
Xenopus
Protein kinase-C Biophysics Agelenopsis aperta Cerebellar mRNA Biochemistry Structural Biology Cerebellum Genetics medicine Staurosporine Animals RNA Messenger Cloning Molecular Molecular Biology Protein kinase C Protein Kinase C Diacylglycerol kinase P-type Ca channel biology Long-term potentiation Cell Biology biology.organism_classification Calcium Channel Blockers Molecular biology Rats Electrophysiology Enzyme Activation Oocytes Phosphorylation Calcium Channels Xenopus oocyte Intracellular medicine.drug |
| Zdroj: | FEBS letters. 317(1-2) |
| ISSN: | 0014-5793 |
| Popis: | Xenopus oocytes injected with rat cerebellar mRNA expressed functional voltage-dependent Ca channels detected as an inward Ba current (IBa). The pharmacological resistance to dihydropyridines and omega-conotoxin together with the blockade obtained with Agelenopsis aperta venom suggest that these channels could be somehow assimilated to P-type Ca channels. The precise nature of the transplanted Ca channels was assessed by hybrid-arrest experiments using a specific oligonucleotide antisense-derivated from the recently cloned alpha 1-subunit of P channels (BI-1 clone). In addition, we demonstrate that exogenous Ca channel activity was enhanced by two different PKC activators (a phorbol ester and a structural analog to diacylglycerol). The general electrophysiological and pharmacological properties of the stimulated Ca channels remain unchanged. This potentiation induced by PKC activators is antagonized by a PKC inhibitor (staurosporine) and by a monoclonal antibody directed against PKC. It is concluded that P-type Ca channels are potentially regulated by PKC phosphorylation and the functional relevance of this intracellular pathway is discussed. |
| Databáze: | OpenAIRE |
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