CRYPTOSPORIDIUM SPP. FROM SMALL MAMMALS IN THE NEW YORK CITY WATERSHED
| Autor: | Susan E. Wade, Stephanie L. Schaaf, Peter E. Ziegler, Hussni O. Mohammed, Yung-Fu Chang |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Veterinary medicine
Watershed Genotype animal diseases Molecular Sequence Data Wildlife Cryptosporidiosis Cryptosporidium Animals Wild Locus (genetics) Polymerase Chain Reaction law.invention Feces Risk Factors Water Supply law Zoonoses parasitic diseases RNA Ribosomal 18S Animals Ecology Evolution Behavior and Systematics Polymerase chain reaction Cryptosporidium parvum Mammals Ecology biology Water Sequence Analysis DNA DNA Protozoan Ribosomal RNA biology.organism_classification New York City Seasons |
| Zdroj: | Journal of Wildlife Diseases. 43:586-596 |
| ISSN: | 0090-3558 |
| DOI: | 10.7589/0090-3558-43.4.586 |
| Popis: | The objective of this study was to assess the potential role that wildlife plays in environmental degradation of watersheds through the contamination of the water supply with zoonotic genotypes of Cryptosporidium. Cryptosporidium isolates recovered from wildlife in the New York City (NYC) watershed were examined to determine genotype using a polymerase chain reaction protocol targeting the 18-Small Subunit (SSU) rRNA locus. Seventy-seven DNA samples recovered from 12 wildlife host species captured in the NYC watershed were amplified and sequenced. Data on risk factors associated with the perpetuation of these genotypes also were collected and analyzed. Although many genotypes appeared to be host-specific, 38% of the samples examined were identified as Cryptosporidium parvum, indicating the presence of zoonotic Cryptosporidium. Adult animals were more likely to shed the zoonotic strains of Cryptosporidium spp. Animals captured in the fall and winter were more likely to be infected with C. parvum than those captured in spring and summer. |
| Databáze: | OpenAIRE |
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