F(ab′)2 anti-CD4 and intact anti-CD4 monoclonal antibodies inhibit the accumulation of CD4+ T cells, CD8+ T cells, and B cells in the kidneys of lupus-prone NZB/NZW mice
| Autor: | Cheryl Schimenti, N L Carteron, Thomas H. Ermak, David Wofsy |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes medicine.medical_specialty medicine.drug_class CD8 Antigens T-Lymphocytes T cell Immunology Biology Kidney Monoclonal antibody Pathology and Forensic Medicine Immunoglobulin Fab Fragments Mice Interleukin 21 Internal medicine medicine Animals Lupus Erythematosus Systemic Immunology and Allergy Cytotoxic T cell B-Lymphocytes Mice Inbred NZB Antibodies Monoclonal T lymphocyte medicine.disease Molecular biology medicine.anatomical_structure Endocrinology CD4 Antigens Female Infiltration (medical) CD8 |
| Zdroj: | Clinical Immunology and Immunopathology. 56:373-383 |
| ISSN: | 0090-1229 |
| Popis: | Murine lupus in NZB/NZW ( B W ) mice is characterized by immune-complex glomerulonephritis and lymphocytic infiltration of several organs, including the kidney. We recently showed that treatment of B W mice with F(ab′)2 anti-CD4 monoclonal antibody retards autoimmunity by inhibiting the function of CD4+ cells and not by depleting them. To determine if treatment with F(ab′)2 anti-CD4 prevented lymphocytic infiltration of kidneys or simply inhibited the function of the infiltrating lymphocytes, long-term survivors of treatment with F(ab′)2 anti-CD4 and intact anti-CD4 were sacrificed for immunohistochemical analysis of their kidneys. Untreated B W mice had large lymphocytic aggregates under the surface epithelium of the renal calyces. Most of these lymphocytes were CD4+ T cells, but CD8+ T cells and B cells were also present. In contrast, treatment with either intact anti-CD4 or F(ab′)2 anti-CD4 substantially reduced, and in many cases prevented, the development of renal infiltrates. Treatment with either form of anti-CD4 not only inhibited renal infiltration by CD4+ T cells, but also prevented the accumulation of CD8+ T cells and B cells. These observations suggest a role for the CD4+ T cell in the accumulation of lymphocytes in target organs. |
| Databáze: | OpenAIRE |
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