The regulatory C proteins from different restriction-modification systems can cross-complement
| Autor: | A Sohail, Joan E. Brooks, Catherine L. Ives |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Transcription
Genetic Blotting Western Bacillus Cross Reactions Microbiology DNA Restriction-Modification Enzymes Cell-free system Bacterial Proteins Species Specificity Genes Regulator Protein biosynthesis Proteus vulgaris Deoxyribonucleases Type II Site-Specific Molecular Biology Gene biology Cell-Free System Deoxyribonuclease BamHI Genetic Complementation Test Molecular biology Biochemistry Polyclonal antibodies Genes Bacterial Protein Biosynthesis biology.protein BamHI Antibody Research Article |
| Popis: | The BamHI restriction-modification system contains a third gene, bamHIC, which positively regulates bamHIR. Similar small genes from other systems were tested in vivo for their ability to cross-complement. C.BamHI protein was identified, purified, and used to raise polyclonal antibodies. Attempts to detect other C proteins in cell extracts by cross-reactivity with C.BamHI antibodies proved unsuccessful. |
| Databáze: | OpenAIRE |
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