| Autor: |
Yangbing Zhao, Carl H. June, Regina M. Young, Andreas Loew, Li Zhou, Brian Granda, Melissa Ramones, Na Li, Alexandria P. Cogdill, Edward C. Pequignot, Devvora Olalere, Shiyu Yang, Chongyun Fang, Shuguang Jiang, Xiaojun Liu |
| Rok vydání: |
2023 |
| DOI: |
10.1158/0008-5472.22405790.v1 |
| Popis: |
Supplementary Table 1, Figures 1-5. Supplemental Table 1 (Table S1) - Affinity of a bivalent antibody (IgG KD) is largely different from it monovalent form (scFv KD). Supplemental Figure 1 (Fig. S1) - Surface expression level of ErbB2 detected by flow cytometry is correlated with mRNA level detected by quantitative PCR. Supplemental Figure 2 (Fig. S2) - CD107a up-regulation was different between higher affinity ErbB2 CART cells and lower affinity CART cells when stimulated with tumor cell lines expressing ErbB2 at different levels. Supplemental Figure 3 (Fig. S3) - More tumor cell lines tested in a CD107a assay confirmed that lower affinity ErbB2 CART cells only recognized ErbB2 over expression tumors. Supplemental Figure 4 (Fig. S4) - ErbB2 target dependent upregulation of CD107a of lower affinity ErbB2 CAR lentiviral transduced, as well as lower affinity ErbB2 CAR mRNA electroporated T cells. Supplemental Figure 5 (Fig. S5) - ErbB2 CART cells with decreased affinity controlled tumor growth as efficiently as ErbB2 CART cells with higher affinity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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